Merck
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N3520

Sigma-Aldrich

Nutrient Mixture F-12 Ham

Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):
Ham’s F-12
Número MDL:
NACRES:
NA.75

esterilidade

sterile

forma

powder

technique(s)

cell culture | mammalian: suitable

componentes

sodium pyruvate: 0.22 g/L
L-glutamine: yes
phenol red: yes
NaHCO3: no
HEPES: no

enviado em

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.

Aplicação

Nutrient Mixture F-12 Ham is used for the following applications:
  • HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum
  • Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)
  • Used in media for the propagation of Cowdria ruminantium
  • Used during preparation of primary mixed glial cultures

Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.

Quantidade

Formulated to contain 11.1 grams of powder per liter of medium.

Reconstituição

Supplement with 2.5 g/L sodium bicarbonate.

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 2

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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