Merck
All Photos(3)

N7653

Sigma-Aldrich

p-Nitrophenyl Phosphate Liquid Substrate System

liquid

All Photos(3)
Synonym(s):
4-Nitrophenyl phosphate disodium salt solution, pNPP
Linear Formula:
O2NC6H4OP(O)(ONa)2
CAS Number:
4264-83-9
Molecular Weight:
263.05
Número MDL:
MFCD00007319
ID de substância PubChem:
24897835
NACRES:
NA.83

forma

liquid

Nível de qualidade

200

enviado em

wet ice

temperatura de armazenamento

−20°C

SMILES string

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI key

VIYFPAMJCJLZKD-UHFFFAOYSA-L

Related Categories

Compare Similar Items

View Full Comparison

1 of 4

This Item
D7679M3168P7127
p-Nitrophenyl Phosphate Liquid Substrate System liquid

Sigma-Aldrich

N7653

p-Nitrophenyl Phosphate Liquid Substrate System

3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System liquid

Sigma-Aldrich

D7679

3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System

4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System liquid

Sigma-Aldrich

M3168

4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System

Phospho(enol)pyruvic acid monopotassium salt ≥97% (enzymatic)

Sigma-Aldrich

P7127

Phospho(enol)pyruvic acid monopotassium salt

form

liquid

form

liquid

form

liquid

form

powder

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Descrição geral

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Aplicação

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Ações bioquímicas/fisiológicas

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Atenção

Stable at least one year when stored below 0 °C.

forma física

Ready-to-use.

Pictogramas

CorrosionHealth hazard
GHS05,GHS08

Palavra indicadora

Danger

Classificações de perigo

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Código de classe de armazenamento

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service