N9287

Sigma-Aldrich

Monoclonal Anti-NBS1 (Nibrin) antibody produced in mouse

~2 mg/mL, clone NBS1-501, purified immunoglobulin, buffered aqueous solution

Sinônimo(s):
Anti-Nijmegen Breakage Syndrome, Anti-p95 Protein of NBS1.MRE11/RAD50 Complex
Número MDL:
NACRES:
NA.41

Nível de qualidade

200

fonte biológica

mouse

forma do anticorpo

purified immunoglobulin

antibody product type

primary antibodies

clone

NBS1-501, monoclonal

forma

buffered aqueous solution

peso molecular

antigen ~95 kDa

species reactivity

human

concentração

~2 mg/mL

aplicação(ões)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2-4 μg/mL using nuclear extract of HEK 293T expressing recombinant human NBS1

Isotipo

IgG1

conjugado

unconjugated

nº de adesão UniProt

enviado em

dry ice

temperatura de armazenamento

−20°C

Gene Information

human ... NBN(4683)

Descrição geral

Monoclonal Anti-NBS1 (Nibrin) (mouse IgG1isotype) is derived from the hybridoma NBS1-501 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human NBS1. Nibrin is mapped to human chromosome 8q21.3. The encoded protein has two domains found in the cell cycle checkpoint proteins, forkhead-associated domain (FHA), and an adjacent breast cancer carboxy-terminal domain (BRCT).

Imunogênio

synthetic peptide corresponding to amino acids 206-220 of human NBS1.

Aplicação

Monoclonal Anti-NBS1 (Nibrin) antibody produced in mouse has been used in:
  • antibody microarray
  • immunocytochemistry
  • immunoblotting
  • immunoprecipitation
  • enzyme linked immunosorbent assay (ELISA)

Ações bioquímicas/fisiológicas

NBS1(Nibrin) was first isolated as a protein involved in DNA repair through analysis of mutations in patients with this syndrome. p95/NBS1(Nibrin) deficiency abrogates the formation of the Meiotic recombination 11 homolog 1(MRE11) / DNA repair protein RAD50 ionizing radiation-induced foci, revealing a molecular link between double-stranded break repair (DSB) and cell cycle checkpoint functions. The phenotypic similarities between ataxia-telangiectasia (AT) and Nijmegen breakage syndrome had suggested that ataxia-telangiectasia mutated gene (ATM) and NBS1 functions in a common signaling pathway. This was confirmed by the finding that in response to ionizing radiation, NBS1is phosphorylated in Ser343 in an ataxia-telangiectasia mutated ATM-dependent manner.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Alemanha

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

Certificado de origem

Identification of intracellular proteins and signaling pathways in human endothelial cells regulated by angiotensin-(1-7)
Meinert C, et al.
Journal of proteomics, 130(4), 129-139 (2016)
Nijmegen breakage syndrome gene (NBS1) is not the tumor suppressor gene at 8q21. 3 involved in colorectal carcinoma
Varon R, et al.
Oncology Reports, 9(4), 709-711 (2002)
ATM phosphorylates p95/nbs1 in an S-phase checkpoint pathway
Lim DS, et al.
Nature, 404(6778), 613-613 (2000)
Further intracellular proteins and signaling pathways regulated by angiotensin-(1-7) in human endothelial cells
Meinert C, et al.
Data in Brief, 10, 354-363 (2017)
Michal Goldberg et al.
Nature, 421(6926), 952-956 (2003-02-28)
MRE11, RAD50 and NBS1 form a highly conserved protein complex (the MRE11 complex) that is involved in the detection, signalling and repair of DNA damage. We identify MDC1 (KIAA0170/NFBD1), a protein that contains a forkhead-associated (FHA) domain and two BRCA1...

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