Merck
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NA1020

Sigma-Aldrich

GenElute PCR Clean-Up Kit

sufficient for 70 purifications

Synonym(s):
Gen Elute, PCR Purification
NACRES:
NA.52

uso

sufficient for 70 purifications

Nível de qualidade

200

temperatura de armazenamento

15-25°C

Descrição geral

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Aplicação

GenElute PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.[1][2][3]
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Características e benefícios

  • Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
  • Recovers up to 95% of PCR products between 100 bp and 10 kb
  • Removes over 99% of primers and other components
  • Eliminates the need to remove mineral oil by organic extraction
  • 40% more purification preps supplied than market leader

Outras notas

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Princípio

The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed).

Informações legais

GenElute is a trademark of Sigma-Aldrich Co. LLC

Pictogramas

CorrosionExclamation mark

Palavra indicadora

Warning

Classificações de perigo

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Central nervous system

Código de classe de armazenamento

8A - Combustible, corrosive hazardous materials

WGK

WGK 2

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificate of Analysis

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Certificate of Origin

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