Merck
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P2088

Sigma-Aldrich

Peroxidase from horseradish

Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)

Synonym(s):
Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase
CAS Number:
Enzyme Commission number:
Número EC:
Número MDL:
NACRES:
NA.54

fonte biológica

horseradish

Nível de qualidade

300

forma

essentially salt-free, lyophilized powder

specific activity

200-300 units/mg solid (using pyrogallol)

peso molecular

~44 kDa

razão de absorbância

RZ 2.6-3.4

temperatura de armazenamento

2-8°C

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Descrição geral

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Aplicação

Peroxidase from horseradish has been used in a study with dehydrogenative polymers (DHP) to investigate the monolignol coupling mechanism. It has also been used to help develop a colorimetric method for microRNA (miRNA) detection by creating a horseradish peroxidase-mimicking DNAzyme.

Embalagem

5000, 10000 units in glass bottle

Ações bioquímicas/fisiológicas

Horseradish peroxidase has been shown to catalyze the polymerization of various substances including acetaminophen and cardanol.

Atenção

Maintains activity at low pH and higher temperature.

Definição da unidade

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Nota de preparo

Stabilized by chemical protection of the primary amines.

Nota de análise

The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Outras notas

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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