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P3627

Sigma-Aldrich

Phosphatase, Acid from wheat germ

≥0.4 unit/mg solid

Todas as fotos(3)
Sinônimo(s):
(Orthophosphoric-monoester phosphohydrolase [acid optimum] ), Acid Phosphatase from wheat germ
Número CAS:
9001-77-8
Número da licença da enzima:
3.1.3.2 (BRENDA, IUBMB)
Número EC:
232-630-9
Número MDL:
MFCD00131847
eCl@ss:
42010102
NACRES:
NA.54

forma

powder

specific activity

≥0.4 unit/mg solid

peso molecular

58 kDa by gel filtration

temperatura de armazenamento

−20°C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

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Este Item
P1146P3752SRE0048
Phosphatase, Acid from wheat germ ≥0.4 unit/mg solid

Sigma-Aldrich

P3627

Phosphatase, Acid from wheat germ

Phosphatase, Acid from potato lyophilized powder, ≥3.0 units/mg solid

Sigma-Aldrich

P1146

Phosphatase, Acid from potato

Phosphatase, Acid from potato lyophilized powder, ≥0.5 unit/mg solid

Sigma-Aldrich

P3752

Phosphatase, Acid from potato

Sigma-Aldrich

Sigma-Aldrich

SRE0048

Phosphatase, Acid from potato

specific activity

≥0.4 unit/mg solid

specific activity

≥3.0 units/mg solid

specific activity

≥0.5 unit/mg solid

specific activity

3.0-10.0 unit/mg solid

mol wt

58 kDa by gel filtration

mol wt

69 kDa

mol wt

69 kDa

mol wt

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

400

Aplicação

Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Product P3627 is from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining.

Ações bioquímicas/fisiológicas

Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes. The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.

Qualidade

Contains lipase

Definição da unidade

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Nota de preparo

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Os números dos produtos são combinados com os tamanhos/quantidades da embalagem quando exibidos no site (exemplo: T1503-25G). Por favor, certifique-se você insere APENAS o número do produto no campo Número do produto (Exemplo T1503).

Exemplo

T1503
Número do produto
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25G
Tamanho/quantidade da embalagem

Exemplos adicionais:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

insira como1.000309185)

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Zuzana Novotná et al.
FEBS letters, 554(1-2), 50-54 (2003-11-05)
Phospholipase D (PLD) forms the major family of phospholipases that was first discovered and cloned in plants. In this report we have shown, for the first time, that C2 phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent PLD(s) from 5 day hypocotyls of Brassica oleracea associated
Yueming Liang et al.
Frontiers in microbiology, 11, 571209-571209 (2020-12-18)
phoD-harboring microorganisms facilitate mineralization of organic phosphorus (P), while their role in the regulation of soil P turnover under P-limited conditions in Pinus massoniana plantations is poorly understood. The aim of the present study was to investigate the effects of
H G Yu et al.
The Journal of cell biology, 145(3), 425-435 (1999-05-04)
We have identified a maize homologue of yeast MAD2, an essential component in the spindle checkpoint pathway that ensures metaphase is complete before anaphase begins. Combined immunolocalization of MAD2 and a recently cloned maize CENPC homologue indicates that MAD2 localizes
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Journal of investigative surgery : the official journal of the Academy of Surgical Research, 26(1), 30-35 (2013-01-01)
Osteoporosis is characterized by poor bone quality. However, it is still controversially discussed whether osteoporosis compromises fracture healing. Herein, we studied whether the course of healing of a femur fracture is affected by osteoporosis or age. Using the senescence-accelerated osteoporotic
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The diagnosis of visceral leishmaniasis (VL) in humans and animal reservoir hosts is difficult, particularly in rural areas where the disease is endemic and laboratory facilities are limited. This study aimed to develop a latex agglutination test (LAT) for the
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Protocolos

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

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