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Phosphatase, Acid from wheat germ

≥0.4 unit/mg solid

Acid Phosphatase from wheat germ, (Orthophosphoric-monoester phosphohydrolase [acid optimum] )
Número CAS:
Número da licença da enzima:
Número EC:
Número MDL:



Nível de qualidade


specific activity

≥0.4 unit/mg solid

peso molecular

58 kDa by gel filtration

temperatura de armazenamento


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Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Product P3627 is from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining.


1, 5, 10 g in poly bottle

Ações bioquímicas/fisiológicas

Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes. The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.


Contains lipase

Definição da unidade

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Nota de preparo

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Storage Class Code

13 - Non Combustible Solids

WGK Alemanha


Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

Certificado de origem

H G Yu et al.
The Journal of cell biology, 145(3), 425-435 (1999-05-04)
We have identified a maize homologue of yeast MAD2, an essential component in the spindle checkpoint pathway that ensures metaphase is complete before anaphase begins. Combined immunolocalization of MAD2 and a recently cloned maize CENPC homologue indicates that MAD2 localizes...
Zuzana Novotná et al.
FEBS letters, 554(1-2), 50-54 (2003-11-05)
Phospholipase D (PLD) forms the major family of phospholipases that was first discovered and cloned in plants. In this report we have shown, for the first time, that C2 phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent PLD(s) from 5 day hypocotyls of Brassica oleracea associated...
Yueming Liang et al.
Frontiers in microbiology, 11, 571209-571209 (2020-12-18)
phoD-harboring microorganisms facilitate mineralization of organic phosphorus (P), while their role in the regulation of soil P turnover under P-limited conditions in Pinus massoniana plantations is poorly understood. The aim of the present study was to investigate the effects of...
Tina Histing et al.
Journal of investigative surgery : the official journal of the Academy of Surgical Research, 26(1), 30-35 (2013-01-01)
Osteoporosis is characterized by poor bone quality. However, it is still controversially discussed whether osteoporosis compromises fracture healing. Herein, we studied whether the course of healing of a femur fracture is affected by osteoporosis or age. Using the senescence-accelerated osteoporotic...
Zhenjie Zhang et al.
International journal of biological macromolecules, 54, 136-143 (2012-12-19)
Purified Rehmannia glutinosa polysaccharide (RGP) is used as functional foods for the prevention and treatment of various diseases. In this study, we examined the effects of RGP on phenotypic and functional maturation of murine bone marrow derived Dendritic cells (BMDCs)....


Enzymatic Assay of Acid Phosphatase (EC

Enzymatic Assay of Acid Phosphatase (EC

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