P3752

Sigma-Aldrich

Phosphatase, Acid from potato

lyophilized powder, ≥0.5 unit/mg solid

Sinônimo(s):
(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato
Número CAS:
Número da licença da enzima:
Número EC:
Número MDL:
eCl@ss:
42010102
NACRES:
NA.54

forma

lyophilized powder

Nível de qualidade

200

specific activity

≥0.5 unit/mg solid

peso molecular

69 kDa

Setor em destaque

Diagnostic Assay Manufacturing

temperatura de armazenamento

−20°C

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Aplicação

Acid phosphatase is routinely used to dephosphorylate proteins and nucleic acids. Acid phosphatase is an acid hydrolase that normally resides in lysosomes. It is used as marker for the identification of lysosomes in subcellular fractionations. Acid phosphatase, product P3752, has been used as a control in order to study how Mycobacterium bovis BCG effects the levels of acid phosphatase activity in alveolar macrophages.

Embalagem

100 mg in poly bottle
1 g in poly bottle

Ações bioquímicas/fisiológicas

Acid phosphatase is a cytoplasmic lysosomal hydrolase that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate.

Definição da unidade

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

RIDADR

NONH for all modes of transport

WGK Alemanha

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

Certificado de origem

I N Mbawuike et al.
Infection and immunity, 51(2), 483-489 (1986-02-01)
The kinetics of induction of the bronchoalveolar cell population (i.e., alveolar macrophages [AM], lymphocytes, and polymorphonuclear leukocytes) was studied in mice inoculated intravenously with heat-killed Mycobacterium bovis BCG. Injection of BCG at 100 and 500 micrograms but not at 10...
Tracy A Briggs et al.
Nature genetics, 43(2), 127-131 (2011-01-11)
We studied ten individuals from eight families showing features consistent with the immuno-osseous dysplasia spondyloenchondrodysplasia. Of particular note was the diverse spectrum of autoimmune phenotypes observed in these individuals (cases), including systemic lupus erythematosus, Sjögren's syndrome, hemolytic anemia, thrombocytopenia, hypothyroidism...
Ekkehart Lausch et al.
Nature genetics, 43(2), 132-137 (2011-01-11)
Vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases are the hallmarks of the genetic disorder spondyloenchondrodysplasia. We mapped a locus in five consanguineous families to chromosome 19p13 and identified mutations in ACP5, which encodes...
Jan Münch et al.
Cell, 131(6), 1059-1071 (2007-12-18)
Sexual intercourse is the major route of HIV transmission. To identify endogenous factors that affect the efficiency of sexual viral transmission, we screened a complex peptide/protein library derived from human semen. We show that naturally occurring fragments of the abundant...
Bernardo de Souza et al.
Inorganic chemistry, 52(7), 3594-3596 (2013-03-19)
Herein we describe the synthesis of a new heterodinuclear Fe(III)Cu(II) model complex for the active site of purple acid phosphatases and its binding to a polyamine chain, a model for the amino acid residues around the active site. The properties...
Protocolos
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
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