P4333

Sigma-Aldrich

Penicillin-Streptomycin

Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture

Sinônimo(s):
Pen-Strep
NACRES:
NA.76

Nível de qualidade

300

esterilidade

0.1 μm filtered

linha de produto

BioReagent

forma

liquid

concentração

100 ×

aplicação(ões)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

Modo de ação

cell wall synthesis | interferes
protein synthesis | interferes

espectro de atividade do antibiótico

Gram-negative bacteria
Gram-positive bacteria

enviado em

dry ice

temperatura de armazenamento

−20°C

SMILES string

CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(O)=O.CNC[C@H]4[C@H](O)[C@@H](O)[C@H](CCO)O[C@H]4O[C@H]5[C@@H](O[C@@H](C)C5(O)C=O)O[C@H]6[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]6NC(N)=N

InChI

1S/C23H43N7O12.C16H18N2O4S/c1-7-23(38,6-32)18(42-19-8(5-28-2)12(33)13(34)9(40-19)3-4-31)20(39-7)41-17-11(30-22(26)27)14(35)10(29-21(24)25)15(36)16(17)37;1-16(2)12(15(21)22)18-13(20)11(14(18)23-16)17-10(19)8-9-6-4-3-5-7-9/h6-20,28,31,33-38H,3-5H2,1-2H3,(H4,24,25,29)(H4,26,27,30);3-7,11-12,14H,8H2,1-2H3,(H,17,19)(H,21,22)/t7-,8-,9-,10+,11-,12-,13-,14+,15-,16+,17+,18-,19-,20-,23+;11-,12+,14-/m01/s1

InChI key

KMOOCZWLFBSQCW-XFOLRDBHSA-N

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Descrição geral

Penicillin Streptomycin solution, also known as Pen Strep, is the most commonly used antibiotic solution for the culture of mammalian cells. Penicillin Streptomycin solution is used to maintain sterile conditions during cell culture, and they show few adverse effects on the cultured cells.

Aplicação

Penicillin-Streptomycin is used to supplement cell culture media to control bacterial contamination. It is recommended for use in cell culture applications at 10 mL/L.
Penicillin streptomycin solution has been used as a supplement in different types of media, including RGC (retinal ganglion cells) culture, α-MEM (minimum essential medium) growth medium, Dulbecco′s modified Eagle′s medium (DMEM)/nutrient mixture F-12 Ham, DMEM and DMEM/F-12 + GlutaMax.

Componentes

This product is formulated to contain 10,000 units penicillin and 10 mg streptomycin/mL solubilized in a proprietary citrate buffer.

Outras notas

20ml,100ml
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Storage class (TRGS 510): Non Combustible Liquids

storage_class_code

12 - Non Combustible Liquids

WGK Alemanha

WGK 2

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

Certificado de origem

184: Toxic effects of antibiotics routinely used in cell culture on primary cultures of dissociated mouse brain cells.
Ammon F
Pediatric Research, 10, 900-900 (1976)
David Sabourin et al.
Journal of laboratory automation, 18(3), 212-228 (2012-09-28)
A microfluidic component library for building systems driving parallel or serial microfluidic-based assays is presented. The components are a miniaturized eight-channel peristaltic pump, an eight-channel valve, sample-to-waste liquid management, and interconnections. The library of components was tested by constructing various...
ZhiMin Wang et al.
Transplantation, 103(3), 502-511 (2018-09-14)
Advances in immunosuppressive therapy have significantly improved short-term but not long-term survival of cardiac transplant recipients; this is largely due to severe cardiac allograft vasculopathy (CAV). Glucagon-like peptide-1 receptor (GLP-1R)-based therapy exerts physiological effects on the cardiovascular system in addition...
J H Martínez-Liarte et al.
Pigment cell research, 8(2), 83-88 (1995-04-01)
Penicillin and streptomycin, the most widely used antibiotics in mammalian cell cultures, caused a moderate stimulation in dopa oxidase and tyrosine hydroxylase activities, but a slight inactivation in the dopachrome tautomerase activity of B16/F10 melanoma cells at the routine concentration...
Ian M Evans et al.
Molecular and cellular biology, 31(6), 1174-1185 (2011-01-20)
Neuropilin-1 (NRP1) is a receptor for vascular endothelial growth factor (VEGF) and plays an important role in mediating cell motility. However, the NRP1 signaling pathways important for cell motility are poorly understood. Here we report that p130(Cas) tyrosine phosphorylation is...
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