Phosphate buffered saline (PBS) is an isotonic buffer solution. This water-based salt solution is made up of disodium hydrogen phosphate, sodium chloride and, in some preparations, potassium chloride and potassium dihydrogen phosphate. PBS is generally used in biological research.
It has also been used for the dilution of capture antibody during α-hemolysin (Hla) enzyme-linked immunosorbent assay (ELISA).
Phosphate buffered saline has been used in bis-benzimide accumulation assay.
Sodium chloride, 145 mM (0.85%) in phosphate buffer, 150 mM