Merck
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P7367

Sigma-Aldrich

PNGase F from Elizabethkingia meningoseptica

BioReagent, ≥95% (SDS-PAGE), for proteomics

Todas as fotos(1)
Sinônimo(s):
Peptide N-glycosidase, PNGase F from Flavobacterium meningosepticum, PNGase F from Chryseobacterium meningosepticum, N-Glycosidase F
Número CAS:
83534-39-8
Número da licença da enzima:
3.5.1.52 (BRENDA, IUBMB)
Número MDL:
MFCD00131222
NACRES:
NA.32

Nível de qualidade

200

fonte biológica

bacterial (Elizabethkingia meningoseptica)

grau

for proteomics

linha de produto

BioReagent

teor

≥95% (SDS-PAGE)

forma

powder

prazo de validade

≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml)
≥1 yr at 2‑8 °C
Solution is stable for at least 3 freeze-thaw cycles

peso molecular

~36 kDa

concentração

≥300 units/mL
≥50 units/mL

pH ideal

~8.6

enviado em

wet ice

temperatura de armazenamento

2-8°C

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Descrição geral

Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.

Aplicação

PNGase F from Elizabethkingia meningoseptica has been used:
  • for de-N-glycosylation of Zika20virus E protein
  • to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
  • to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)

Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis
Used to deglycosylate protein.

Ações bioquímicas/fisiológicas

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Definição da unidade

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 2

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

Product Information Sheet

Product Information Sheet - P7367

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Glycoconjugate journal, 35(6), 499-509 (2018-11-24)
Analysis of glycans via a porous graphitized carbon liquid chromatography (PGC-LC) coupled with electrospray ionization (tandem) mass spectrometry (ESI-MS(/MS)) is a powerful analytical method in the field of glycomics. Isobaric glycan structures can be identified reliably with the help of
Katherine J D Ashbourne Excoffon et al.
Journal of virology, 81(11), 5573-5578 (2007-03-23)
The coxsackievirus and adenovirus receptor (CAR) is both a viral receptor and homophilic adhesion protein. The extracellular portion of CAR consists of two immunoglobulin (Ig)-like domains, each with a consensus sequence for N-glycosylation. We used chemical, genetic, and biochemical studies
Lars Andresen et al.
Journal of immunology (Baltimore, Md. : 1950), 188(4), 1847-1855 (2012-01-10)
NKG2D ligand surface expression is important for immune recognition of stressed and neotransformed cells. In this study, we show that surface expression of MICA/B and other NKG2D ligands is dependent on N-linked glycosylation. The inhibitor of glycolysis and N-linked glycosylation
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