Merck
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P9416

Sigma-Aldrich

TWEEN® 20

for molecular biology, viscous liquid

Synonym(s):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
CAS Number:
Número MDL:
NACRES:
NA.31

grau

for molecular biology

Nível de qualidade

300

descrição

non-ionic

teor

≥40.0% (GC)

forma

viscous liquid

peso molecular

~1228
~1228

composição

lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)

índice de refração

n20/D 1.468 (lit.)

CMC

60 mg/L

densidade

1.095 g/mL at 25 °C (lit.)

adequação

suitable for molecular biology

atividade externa

Endonuclease-exonuclease and RNase, none detected

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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Descrição geral

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Aplicação

TWEEN 20 is widely used in biochemical applications. It has been used:
  • As an emulsifying agent for the preparation of stable oil-in-water emulsions
  • In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
  • As a blocking agent for membrane based immunoassays at a typical concentration of 0.05%
  • For lysing mammalian cells at a concentration of 0.005 to 0.5%
  • Along with PBS in the dilution of antibodies in immunohistochemistry technique
  • For washing cells in FISH (Fluorescence in situ hybridization) technique
Non-ionic detergent

Embalagem

50, 100 mL in poly bottle

Atenção

TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.

Nota de preparo

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Informações legais

TWEEN is a registered trademark of Croda International PLC

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 1

Ponto de fulgor (ºF)

527.0 °F - Pensky-Martens closed cup

Ponto de fulgor (ºC)

275 °C - Pensky-Martens closed cup

Equipamento de proteção individual

Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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