Merck
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P9582

Sigma-Aldrich

Polyinosinic–polycytidylic acid potassium salt

with buffer salts, TLR ligand tested

Synonym(s):
Poly (I:C), Poly(I) • Poly(C)
CAS Number:
Número MDL:
NACRES:
NA.51

Nível de qualidade

300

teor

≥99% (less than 1% free nucleotides, TLC)

forma

lyophilized powder

composição

Poly(I) • Poly(C), 10% (balance buffer salts as sodium chloride and sodium phosphate)

condição de armazenamento

desiccated

temperatura de armazenamento

−20°C

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Aplicação

TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.

Embalagem

5, 50 mg in glass bottle
Package size based on polynucleotide content

Ações bioquímicas/fisiológicas

Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.

Outras notas

Double-stranded homopolymer.

Nota de preparo

The product requires ionic strength to maintain the double-strand structure. Reconstitution at ~10 mg/mL of water yields a polynucleotide in physiological phosphate buffered solution.

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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