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PP0200

Sigma-Aldrich

GlycoProfile I, In-Gel Deglycosylation Kit

Número EC:
NACRES:
NA.32

Nível de qualidade

200

prazo de validade

≥1 yr at 2‑8 °C

temperatura de armazenamento

2-8°C

Aplicação

The GlycoProfile Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.

Princípio

The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.

Quantidade

Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.

Informações legais

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS

  • P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 U

  • T6567Trypsin from porcine pancreas, BioReagent, Proteomics Grade, Dimethylated 20 μg

  • Trypsin Solubilization Reagent

  • I0408Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiae, for proteomics

  • Acetonitrile, ≥99.93%, BioSyn® 50 mL

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 3

Certificado de análise

Certificado de origem

Artigos

O-Linked Glycan Strategies

There is no enzyme comparable to PNGase F for removing intact O-linked sugars. Monosaccharides must be sequentially hydrolyzed by a series of exoglycosidases until only the Gal-b(1-3)-GalNAc core remains. O-Glycosidase can then remove the core structure intact with no modification of the serine or threonine residue.

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Deglycosylation Kits

Deglycosylation Kits

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