Merck
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R5886

SAFC

RPMI-1640 Medium

HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture

Sinônimo(s):
Roswell Park Memorial Institute 1640 medium
Número MDL:
NACRES:
NA.75

Nível de qualidade

400

esterilidade

sterile-filtered

forma

liquid

technique(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

pH

>7.2

componentes

L-glutamine: no
NaHCO3: yes
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes

enviado em

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.

Aplicação

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 2

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

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