Merck
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R6750

Sigma-Aldrich

Ribonucleic acid from baker's yeast (S. cerevisiae)

Sinônimo(s):
RNA
Número CAS:
Número MDL:
NACRES:
NA.51

Nível de qualidade

200

temperatura de armazenamento

−20°C

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Descrição geral

Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is the substrate for RNase enzyme. The isolation of RNA from yeast is a complicated process and involves heating and freezing cycles of cells in the presence of phenyl and detergents (SDS).

Aplicação

Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is suitable for applications such as northern blot hybridization, reverse transcriptase-polymerase chain reaction (RTPCR), and cDNA construction.

Embalagem

100, 250, 500 mg in poly bottle
1 g in poly bottle

Nota de preparo

Prepared by a modification of Crestfield, A.M., et al., J. Biol. Chem., 216, 185 (1955).

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

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Certificado de origem

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Kazuya Shirato et al.
Virology journal, 11, 139-139 (2014-08-12)
The first documented case of Middle East Respiratory Syndrome coronavirus (MERS-CoV) occurred in 2012, and outbreaks have continued ever since, mainly in Saudi Arabia. MERS-CoV is primarily diagnosed using a real-time RT-PCR assay, with at least two different genomic targets
Radha Duttagupta et al.
PloS one, 7(2), e31241-e31241 (2012-02-24)
Inflammatory Bowel Disease--comprised of Crohn's Disease and Ulcerative Colitis (UC)--is a complex, multi-factorial inflammatory disorder of the gastrointestinal tract. In this study we have explored the utility of naturally occurring circulating miRNAs as potential blood-based biomarkers for non-invasive prediction of
Lukas Bossaller et al.
Journal of immunology (Baltimore, Md. : 1950), 197(4), 1044-1053 (2016-06-30)
Systemic lupus erythematosus (SLE) is a chronic, life-threatening autoimmune disorder, leading to multiple organ pathologies and kidney destruction. Analyses of numerous murine models of spontaneous SLE have revealed a critical role for endosomal TLRs in the production of autoantibodies and
Stephanie C Bannister et al.
Biology of reproduction, 81(1), 165-176 (2009-04-10)
MicroRNAs are a highly conserved class of small RNAs that function in a sequence-specific manner to posttranscriptionally regulate gene expression. Tissue-specific miRNA expression studies have discovered numerous functions for miRNAs in various aspects of embryogenesis, but a role for miRNAs
Yajuan Qi et al.
Circulation. Heart failure, 8(1), 198-208 (2014-12-06)
Heart failure is a leading cause of morbidity and mortality in the USA and is closely associated with diabetes mellitus. The molecular link between diabetes mellitus and heart failure is incompletely understood. We recently demonstrated that insulin receptor substrates 1

Protocolos

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

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