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Merck

R7256

Sigma-Aldrich

RPMI 1640 Vitamins Solution (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Sinônimo(s):

RPMI 1640 Solution, RPMI 1640 Vitamins, Vitamins Solution

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100 ML
R$ 276,00

R$ 276,00


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Sobre este item

Código UNSPSC:
12352205
NACRES:
NA.75

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esterilidade

sterile-filtered

Nível de qualidade

linha de produto

BioReagent

Formulário

liquid

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

cor

yellow to dark yellow

pH

7.2-7.6

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

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1 of 4

Este Item
FG1215R6504R7131
Sigma-Aldrich

Sigma-Aldrich

R6504

Meio RPMI-1640

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

powder

form

liquid

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

dry ice

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

200

Quality Level

400

Quality Level

500

Quality Level

400

color

yellow to dark yellow

color

-

color

-

color

-

Descrição geral

Roswell Park Memorial Institute medium is commonly referred as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. RPMI 1640 is used for the serum-free expansion of human lymphoid cells.

Aplicação

RPMI 1640 Vitamins Solution (100×) has been used:
  • in the preparation of CeM1 medium for the isolation of Caenorhabditis elegans germ cells[1]
  • as a component of the amino-acid-deprived medium for the starvation of intestinal porcine enterocytes[2]
  • as a component of the stage-8 medium for culturing of human pluripotent stem cell-derived 3D pancreatic progenitor clusters[3]
RPMI 1640 Vitamins Solution (100×) is used as a supplement in cell culture media.[4][5][6][7]

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Leucine stimulates ASCT2 amino acid transporter expression in porcine jejunal epithelial cell line (IPEC-J2) through PI3K/Akt/mTOR and ERK signaling pathways
Shihai Z, et al.
Amino Acids, 46(12) (2014)
Nieves Vizcaíno et al.
Veterinary research, 51(1), 101-101 (2020-08-17)
Brucella ovis is a non-zoonotic rough Brucella that causes genital lesions, abortions and increased perinatal mortality in sheep and is responsible for important economic losses worldwide. Research on virulence factors of B. ovis is necessary for deciphering the mechanisms that
Joshua R Kane et al.
eLife, 9 (2020-01-22)
In pursuit of therapeutics for human polyomaviruses, we identified a peptide derived from the BK polyomavirus (BKV) minor structural proteins VP2/3 that is a potent inhibitor of BKV infection with no observable cellular toxicity. The thirteen-residue peptide binds to major
Indranil Biswas et al.
Journal of bacteriology, 189(18), 6521-6531 (2007-07-10)
Streptococcus mutans, the principal causative agent of dental caries, produces four glucan-binding proteins (Gbp) that play major roles in bacterial adherence and pathogenesis. One of these proteins, GbpC, is an important cell surface protein involved in biofilm formation. GbpC is
Alexandra S Vagasi et al.
Bio-protocol, 7(15) (2017-09-05)
The Caenorhabditis elegans germ line is an important model system for the study of germ stem cells. Wild-type C. elegans germ cells are syncytial and therefore cannot be isolated in in vitro cultures. In contrast, the germ cells from tumorous

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Questions

  1. Is it possible to know the full listing of the components of RPMI 1640 100X vitamins? We would like to know if it is an appropriate additive to media we are developing for growth of oral biofilms.

    1 answer
    1. The formulation of Product No. R7256, RPMI 1640 Vitamins Solution (100×) can be found at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/343/805/r7256for.pdf

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