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S5395

Sigma-Aldrich

Superoxide Dismutase from bovine erythrocytes

≥3,000 units/mg protein, BioReagent, lyophilized powder, suitable for cell culture

Synonym(s):
SOD, Superoxide: superoxide oxidoreductase
CAS Number:
Enzyme Commission number:
Número EC:
Número MDL:
NACRES:
NA.75

fonte biológica

bovine erythrocytes

Nível de qualidade

200

linha de produto

BioReagent

forma

lyophilized powder

specific activity

≥3,000 units/mg protein

peso molecular

32.5 kDa

embalagem

pkg of 15000 units

technique(s)

cell culture | mammalian: suitable

pH

7.6-10.5

enviado em

dry ice

temperatura de armazenamento

−20°C

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Descrição geral

Superoxide Dismutase from bovine erythrocytes is a metalloprotein which disproportionates superoxide anion radicals. It is a 31.5 kDa copper binding protein and displays a conserved domain and fold. It is a homodimer with one copper and zinc ion per subunit and has antiparallel “greek-key” β barrel fold.

Aplicação

Superoxide Dismutase (SOD) from bovine erythrocytes has been used:
  • for measuring the superoxide radical using the electron paramagnetic resonance spin in human brain microvascular endothelial cells
  • for measuring superoxide production in cytochrome C assay in peripheral blood mononuclear cells
  • as a standard in characterization of hen egg SOD using Fourier-transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption/ionization (MALDI) analysis

Embalagem

15000 units in glass bottle
30000, 75000 units in poly bottle

Ações bioquímicas/fisiológicas

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Definição da unidade

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

Nota de análise

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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