Merck
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S6639

Sigma-Aldrich

SSC Buffer 20× Concentrate

for Northern and Southern blotting, solution

All Photos(1)
Synonym(s):
20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer
NACRES:
NA.25

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Denhardt′s Solution 50x

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for molecular biology

Nível de qualidade

200

descrição

Saline-Sodium Citrate buffer, made with ultrapure water

sterility

0.2 μm filtered

forma

solution

application(s)

diagnostic assay manufacturing

atividade externa

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temperatura de armazenamento

room temp

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Descrição geral

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.

Aplicação

SSC Buffer 20× Concentrate has been used
  • as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
  • as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
  • as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon

Embalagem

1, 1 L

Características e benefícios

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Componentes

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificate of Analysis

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Certificate of Origin

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