S9137

Streptomycin sulfate salt

powder, BioReagent, suitable for cell culture

• Fórmula linear: C₂₁H₃₉N₇O₁₂ · 1.5 H₂SO₄
• Número CAS: 3810-74-0
• Peso molecular: 728.69
• Beilstein: 3894995
• Número EC: 223-286-0
• Número MDL: MFCD00037023
• ID de substância PubChem: 24899828
• NACRES: NA.76

Propriedades

• Nível de qualidade: 200
• linha de produto: BioReagent
• forma: powder
• potência: ≥720 I.U. per mg (dried basis)
• condição de armazenamento: (Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)
• technique(s): cell culture | mammalian: suitable
• cor: white to off-white
• espectro de atividade do antibiótico: Gram-negative bacteria; Gram-positive bacteria; mycobacteria
• Modo de ação: protein synthesis | interferes
• temperatura de armazenamento: 2-8°C
• SMILES string: OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@H]2[C@@H](O[C@@H](C)[C@]2(O)C=O)O[C@@H]3[C@@H](O)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]3NC(N)=N.CN[C@H]4[C@H](O)[C@@H](O)[C@H](CO)O[C@H]4O[C@H]5[C@@H](O[C@@H](C)[C@]5(O)C=O)O[C@@H]6[C@@H](O)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]6NC(N)=N
• InChI: 1S/2C21H39N7O12.3H2O4S/c2*1-5-21(36,4-30)16(40-17-9(26-2)13(34)10(31)6(3-29)38-17)18(37-5)39-15-8(28-20(24)25)11(32)7(27-19(22)23)12(33)14(15)35;3*1-5(2,3)4/h2*4-18,26,29,31-36H,3H2,1-2H3,(H4,22,23,27)(H4,24,25,28);3*(H2,1,2,3,4)/t2*5-,6-,7-,8+,9-,10-,11-,12+,13-,14-,15-,16-,17-,18-,21+;;;/m00.../s1
• InChI key: QTENRWWVYAAPBI-YZTFXSNBSA-N

Descrição

Descrição geral

Chemical structure: aminoglycoside

Embalagem

25, 100 g in poly bottle

Aplicação

Streptomycin is used to study mechanisms of streptomycin resistance and is often used together with penicillin and other agents to inhibit bacterial contamination in cell culture applications. It is recommended for use in molecular biology applications at 25-50μg/mL, in cell culture applications at 100 mg/L and in embryo culture at 50 mg/L.

Ações bioquímicas/fisiológicas

Mode of Action: Streptomycin acts by inhibiting prokaryote protein synthesis by binding to the S12 protein of the 30S ribosomal subunit, thus preventing the transition from imitation complex to chain-elongating ribosome. This causes miscoding or inhibits initiation.

Mode of Resistance: A mutation in rpsL, a gene for S12 ribosomal protein, prevents binding of streptomycin to the ribosome. An aminoglycoside phosphotransferase also inactivates streptomycin.

Antimicrobial spectrum: Streptomycin acts against gram-negative and gram-positive bacteria.

Atenção

Solutions can be stored at 2-8°C for up to a month or at -20°C for more extended periods. At 37°C, solutions are stable for three days.

Nota de preparo

Stock solutions should be prepared directly in the vial with sterile water at concentrations between 20-25 mg/mL.

Outras notas

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place

Informações de segurança

• Pictogramas: GHS07,GHS08
• Palavra indicadora: Warning
• Frases de perigo: H302 - H361fd
• Declarações de precaução: P201 - P202 - P264 - P270 - P301 + P312 - P308 + P313
• Classificações de perigo: Acute Tox. 4 Oral - Repr. 2
• Código de classe de armazenamento: 11 - Combustible Solids
• WGK: WGK 3
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Faceshields, Gloves