Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos

SAB4200776

Sigma-Aldrich

Anti-Tubulin, Tyrosine antibody, Mouse monoclonal

clone TUB-1A2, purified from hybridoma cell culture

Sinônimo(s):

Tubulin Alpha-1 Chain

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified from hybridoma cell culture

tipo de produto de anticorpo

primary antibodies

clone

TUB-1A2, monoclonal

forma

buffered aqueous solution

peso molecular

~55 kDa

reatividade de espécies

Xenopus, Arabidopsis sp., yeast, insect, rat, hamster, porcine, monkey, bovine, human, mouse, canine, chicken

concentração

~1 mg/mL

técnica(s)

immunoblotting: suitable
immunofluorescence: 2-4 μg/mL using human HeLa or Chicken fibroblast cells
immunohistochemistry: suitable

Isotipo

IgG3

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... TUBA1A(7846)

Categorias relacionadas

Descrição geral

Anti-Tubulin, Tyrosine antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the TUB-1A2 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse. Tubulin is an intracellular cylindrical filamentous structure and is presented in eukaryotic cells. It consists of three isoforms α-, β- and γ-tubulin. Tubulin is a heterodimer of α- and β- tubulin subunits.

Especificidade

Anti-Tubulin, Tyrosine antibody, Mouse monoclonal specifically recognizes the C-terminal Tyrosine of Tubulin protein. The antibody is non-reactive with cells that have been treated with pancreatic carboxypeptidase A under conditions which remove only the C-terminal tyrosine. Monoclonal Anti-Tubulin, Tyrosine recognizes Tubulin from human, mouse, chicken, bovine, rat, hamster, canine, monkey, porcine, arabidopsis, tobacco, yeast, Xenopus, insect, comb jelly, sponge, and worm origin.

Imunogênio

a synthetic peptide containing the carboxy terminal amino acids of porcine a-tubulin

Aplicação

Anti-Tubulin, Tyrosine antibody, mouse monoclonal may be used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Ações bioquímicas/fisiológicas

Tubulin is the major building block of microtubules which is essential for structural and mobility elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. It is also involved in the nervous system. Tubulin is associated with neurodegenerative disorders such as Alzheimer′s and Parkinson′s disease. Mutations in the αβ- dimers and γ-tubulin are associated with malformation and impairment of cognitive disorders.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Ferramenta de seleção de produtos.

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Tubulin: Structure, Functions and Roles in Disease
Binarova P and Tuszynski J
Cells, 10(3), 194-204 (2019)
Microtubules and resistance to tubulin-binding agents
Kavallaris M
Nature Reviews. Cancer, 10(3), 194-204 (2010)

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica