Merck
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SAB4301138

Sigma-Aldrich

Anti-GFP antibody produced in rabbit

affinity isolated antibody

Synonym(s):
GFP-like chromoprotein
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

100

forma do anticorpo

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

27 kDa

concentração

3.2 mg/mL

technique(s)

western blot: 1:1000-1:10000 (Cell Lysate)

Isotipo

IgG

enviado em

wet ice

temperatura de armazenamento

−20°C

Descrição geral

Green fluorescent protein (GFP) is a 27kDa protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+- activated photoprotein aequorin to GFP.[1][2]

Especificidade

The antibody detects transfected proteins containing GFP tag.

Imunogênio

Full length fusion protein

Aplicação

Anti-GFP antibody produced in rabbit has been used in:
  • double immunofluorescence staining [3]
  • immunoblot analysis.[4]
  • immunoprecipitation.[5]

Ações bioquímicas/fisiológicas

Green fluorescent protein (GFP) is a reporter molecule which is used for checking gene expression and protein localization in vivo, in situ and in real time. GFP emits green light when it is excited with UV/blue light. The GFP fluorescence remains stable and can be detected non-invasively in living cells.[6] GFP is considered as a unique tool to monitor dynamic processes in several living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence.[7]

Características e benefícios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

forma física

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificate of Analysis

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Certificate of Origin

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