SAB5200020

Sigma-Aldrich

Monoclonal Anti-MDC1 antibody produced in mouse

clone P2B11, 1 mg/mL, purified immunoglobulin

Sinônimo(s):
Anti-Nuclear factor with BRCT domains1, Anti-MDC1, P2B11, Anti-mediator of DNA damage checkpoint 1
NACRES:
NA.41

Nível de qualidade

100

fonte biológica

mouse

forma do anticorpo

purified immunoglobulin

antibody product type

primary antibodies

clone

P2B11, monoclonal

forma

buffered aqueous glycerol solution

peso molecular

antigen predicted mol wt 184 kDa

species reactivity

human, bovine, mouse, chimpanzee

concentração

1 mg/mL

aplicação(ões)

indirect immunofluorescence: suitable
western blot: suitable

Isotipo

IgG1

conjugado

unconjugated

nº de adesão NCBI

nº de adesão UniProt

enviado em

wet ice

temperatura de armazenamento

−20°C

Gene Information

mouse ... MDC1(240087)

Descrição geral

Mediator of DNA damage checkpoint 1 (MDC1) is a nuclear protein that in humans is encoded by the MDC1 gene mapped to chromosome 6p21.33. The encoded protein is widely expressed in various types of tissues and organs. MDC1 is characterized with two BRCA1 C terminus (BRCT) domain, an N-terminal phosphoamino-acid-binding motif called a forkhead-associated (FHA) domain and large central proline/serine/threonine-rich repeat (PST) domain.

Especificidade

Detects ~184 kDa. This antibody recognizes MDC1 at and around the N-terminus.

Imunogênio

GST-tagged recombinant protein corresponding to mouse MDC1 ar and around the N-termius

Ações bioquímicas/fisiológicas

Mediator of DNA damage checkpoint 1 (MDC1) plays a vital role in stimulation of the intra–S phase and G2-M phase checkpoints of the cell cycle in response to DNA damage. Polymorphism of the gene has been associated with the development of both the serum LDL-cholesterol concentration and hyper–LDL-cholesterolemia. Mammalian MDC1/ nuclear factor with BRCT domain 1 (NFBD1) interacts with phospho-H2AX (γH2AX) and plays a key role in DNA damage response by facilitating normal radioresistance and efficient accumulation of DNA-damage-response proteins on damaged chromatin.

Características e benefícios

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

forma física

Solution in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Alemanha

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificado de análise

Certificado de origem

MDC1 Directly Binds Phosphorylated Histone H2AX to Regulate Cellular Responses to DNA Double-Strand Breaks
Stucki M
Cell, 123, 1213-1226 (2005)
MDC1 is a mediator of the mammalian DNA damage checkpoint
Stewart GS
Nature, 421, 961-966 (2003)
Identification of eight genetic variants as novel determinants of dyslipidemia in Japanese by exome-wide association studies.
Yamada Y
Oncotarget, 8, 38950-38961 (2017)
Yuki Yamamoto et al.
Cancer research, 74(14), 3707-3715 (2014-05-17)
Failure to expeditiously repair DNA at sites of double-strand breaks (DSB) ultimately is an important etiologic factor in cancer development. NBS1 plays an important role in the cellular response to DSB damage. A rare polymorphic variant of NBS1 that resulted...
Bárbara Alcaraz Silva et al.
The Journal of biological chemistry, 289(33), 22771-22784 (2014-07-02)
Chromosome ends contain nucleoprotein structures known as telomeres. Damage to chromosome ends during interphase elicits a DNA damage response (DDR) resulting in cell cycle arrest. However, little is known regarding the signaling from damaged chromosome ends (designated here as "TIPs")...

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