Merck
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SBR00053

Sigma-Aldrich

Vitamin K1 Ready Made Solution

5 mg/mL

forma

liquid

Nível de qualidade

concentração

5 mg/mL

temperatura de armazenamento

2-8°C

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This Item
K3129MBD0043V-041
Vitamin K1-13C6 solution 5 μg/mL in ethanol, certified reference material, ampule of 1 mL, Cerilliant®

Supelco

V-041

Vitamin K1-13C6 solution

form

liquid

form

liquid

form

-

form

liquid

concentration

5 mg/mL

concentration

100 ×

concentration

-100 ×

concentration

5 μg/mL in ethanol

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−70°C

Quality Level

200

Quality Level

400

Quality Level

200

Quality Level

300

Descrição geral

Vitamin K1 (Phylloquinone) is a lipid soluble polycyclic aromatic ketone that is used as a cofactor in the forming of coagulation factors II (prothrombin), VII, IX and X. It is also an anticoagulant factor for protein C and S and as a cell signaling factor . VitaminK1 is synthesized by plants and some cyanobacteria to be used as a redox cofactor, and is converted into vitamin K2 by intestinal microbiota in the human gut. Vitamin K1 has shown to enhance the growth of many anaerobes including Bacteroides species and certain gram-positive non-spore forming organisms . Vitamin K1 a is also a key ingredient compound in certain anaerobic mediums such as: Brucella broth, Thioglycolate broth, reinforced Clostridial Medium, etc. An in vitro study performed by Kijewski, et al., showed that Vitamin K analogs phylloquinone (vitamin K1), menaquinone (vitamin K2), and menadione (vitamin K3), inhibit the growth of Enterohemorrhagic Escherichia coli (EHEC) and the production of its main virulence factor; the Shiga toxin.

Aplicação

For 1 liter (L) of medium preparation, it is recommended to add 0.2 mL Vitamin K Ready Made Solution after autoclaving of medium, to achieve a final concentration of 1 microgram/ml. The optimal concentration of supplement may vary for each strain. Select strains which show turbid growth (24-48 hr) with vitamin K1 supplementation: Porphyromonas gingivalis, Bifidobacterium longu, Clostridium perfringens, Bacteroides vulgatus and Fusobacterium nucleatum.

Armazenamento e estabilidade

1. The solution is classified as light sensitive.

2. The solution cannot be heated and must be added aseptically in proper amounts to media that has been autoclaved and cooled to 50-55 ºC.

Pictogramas

FlameExclamation mark

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Flam. Liq. 2

Código de classe de armazenamento

3 - Flammable liquids

WGK

WGK 1

Ponto de fulgor (ºF)

55.4 °F

Ponto de fulgor (ºC)

13 °C


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