Merck
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SCA20

Supelco

Sucrose Assay Kit

sufficient for 20 assays

Número EC:
NACRES:
NA.84

uso

sufficient for 20 assays

Nível de qualidade

100

analyte chemical class(es)

sugars (sucrose)

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

temperatura de armazenamento

2-8°C

Descrição geral

Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.

Aplicação

This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.

Kit Components Only

Product No.
Description

  • glucose assay reagent 50 mL

  • sucrose assay reagent 1 mL/vial

  • sucrose standard 500 mg

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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