Merck
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SRE0065

Sigma-Aldrich

HEPES solution, 1M

Reagent designed and manufactured under cGMP controls suitable for use in an IVD application

Synonym(s):
HEPES Buffer, N-(2-Hydroxyethyl)piperazineN′-(2-ethanesulfonic acid)

Nível de qualidade

400

forma

liquid

concentração

1 M

cor

colorless

pH

7.2-7.5 (25 °C)

application(s)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

Aplicação

HEPES buffer preserves a normal physiological pH environment and is commonly used as a buffer in cell culture applications. HEPES buffer provides no nutritional benefit to cells, but adds extra buffering capacity to the cell culture particularly during experiments performed outside the carbon dioxide incubator.
This 1 M stock solution contains HEPES adjusted to pH 7.35 with sodium hydroxide. Add the concentrated buffer to the cell culture medium to reach the desired concentration (typically 10–25 mM).

Notes: If precipitation is present in the concentrated buffer, gently warm the bottle to 37 °C and mix until completely dissolved prior to dilution.

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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