Merck
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SRP3093

Sigma-Aldrich

IL-4 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
B cell stimulating factor (BSF-1), BCDF, BCGF
NACRES:
NA.32

fonte biológica

human

recombinante

expressed in E. coli

teor

≥98% (HPLC)
≥98% (SDS-PAGE)

forma

lyophilized

potência

≤0.2 ng/mL ED50

peso molecular

14.9 kDa

embalagem

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

Impurezas

<0.1 EU/μg endotoxin, tested

cor

off-white to yellow

nº de adesão UniProt

enviado em

wet ice

temperatura de armazenamento

−20°C

Gene Information

human ... IL4(3565)

Descrição geral

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues.

Aplicação

Interleukin-4 (IL-4) has been used as a stimulant of M2 state of microglia that is associated with wound healing and debris clearance.

Ações bioquímicas/fisiológicas

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues.

Sequência

MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

forma física

Lyophilized with no additives.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Certificate of Analysis

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Certificate of Origin

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