T1775

L-Thyroxine

powder, BioReagent, suitable for cell culture

• Synonym(s): 3,3′,5,5″-Tetraiodo-L-thyronine, 3-[4-(4-Hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-L-alanine, T₄
• Linear Formula: HOC₆H₂(I)₂OC₆H₂(I)₂CH₂CH(NH₂)CO₂H
• CAS Number: 51-48-9
• Molecular Weight: 776.87
• Beilstein: 2228515
• Número EC: 200-101-1
• Número MDL: MFCD00002595
• eCl@ss: 32190102
• ID de substância PubChem: 24899990
• NACRES: NA.77

Properties

• fonte biológica: synthetic
• Nível de qualidade: 200
• linha de produto: BioReagent
• teor: ≥98% (HPLC)
• forma: powder
• potência: 5-50 ng/mL
• technique(s): cell culture | mammalian: suitable
• pf: 223 °C (dec.) (lit.)
• solubilidade: 4 M NH₄OH in methanol: 50 mg/mL, clear to hazy, yellow
• enviado em: ambient
• temperatura de armazenamento: room temp
• SMILES string: N[C@@H](Cc1cc(I)c(Oc2cc(I)c(O)c(I)c2)c(I)c1)C(O)=O
• InChI: 1S/C15H11I4NO4/c16-8-4-7(5-9(17)13(8)21)24-14-10(18)1-6(2-11(14)19)3-12(20)15(22)23/h1-2,4-5,12,21H,3,20H2,(H,22,23)/t12-/m0/s1
• InChI key: XUIIKFGFIJCVMT-LBPRGKRZSA-N
• Gene Information: human ... THRA(7067) , THRB(7068) , TTR(7276) ; mouse ... Cnr2(12802) ; rat ... Cnr1(25248)

Description

Descrição geral

Thyroxine (T₄) is a tetraiodo derivative of the p-hydroxyphenyl ether of tyrosine, which functions as growth factors or cell growth inhibitors. T₄ is used to treat hypothyroidism and thyroid carcinoma. It regulates energy metabolism.

Aplicação

L-Thyroxine has been used to investigate its role in colorectal cancer cell lines HCT 116 (APC wild type) and HT-29 (APC mutant). It has also been used to evaluate its effects on pro-inflammatory tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), angiogenic vascular endothelial growth factor (VEGF), leukocyte adhesion, intercellular adhesion molecule 1 (ICAM-1) and E-selectin in human umbilical vein endothelial cells (HUVECs).

Ações bioquímicas/fisiológicas

L-Thyroxine (T₄) and triiodo-L-thyronine (T₃) are iodine-containing hormones produced from thyroglobulin in the thyroid follicular cells. The stimulation of metabolic rate and regulation of growth and development by these hormones appear to be due to their effects on DNA transcription and, thus, protein synthesis.

Nota de preparo

For long term storage, keep stock solutions at -20° in working aliquots and avoid repeated freeze/thaw cycles. Solutions stored at 2-8 °C are stable for up to 30 days.

Reconstituição

To prepare 20 μg/ml stock solution, dissolve in 50 ml sterile culture medium per mg thyroxine.

Safety Information

• Código de classe de armazenamento: 13 - Non Combustible Solids
• WGK: WGK 3
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)