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T2885

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

≥98% (TLC)

Sinônimo(s):
TMB substrate, TMB, Sure Blue TMB, BM blue, TMB Blotting Plus
Fórmula linear:
[-C6H2(CH3)2-4-NH2]2
Número CAS:
Peso molecular:
240.34
Beilstein:
2808541
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.83

Nível de qualidade

200

teor

≥98% (TLC)

forma

powder

pf

168-171 °C (lit.)

solubilidade

ethyl acetate: 50 mg/mL, clear to very slightly hazy

temperatura de armazenamento

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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Aplicação

3,3′,5,5′-Tetramethylbenzidine has been used:
  • as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stains the lactobacilli colonies blue in the presence of O2
  • as a substrate solution for ELISA (enzyme-linked immunosorbent assay)
  • in myeloperoxidase activity assay to quantify neutrophil

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Embalagem

100 mg in glass bottle
1, 5, 100 g in glass bottle
Bottomless glass bottle. Contents are inside inserted fused cone.

Ações bioquímicas/fisiológicas

3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificado de análise

Certificado de origem

Lactobacillus casei improves resistance to pneumococcal respiratory infection in malnourished mice
Villena J, et al.
The Journal of Nutrition, 135(6), 1462-1469 (2005)
Tetramethylbenzidine method for monitoring the free available chlorine and microbicidal activity of chlorite-based sanitizers under organic-matter-rich environments
Yamaoka H, et al.
Letters in Applied Microbiology, 62(1), 47-54 (2016)
The CD4+ T cell-mediated IFN-gamma response to Helicobacter infection is essential for clearance and determines gastric cancer risk
Sayi A, et al.
Journal of immunology (Baltimore, Md. : 1950), 182(11), 7085-7101 (2009)
Lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle
Rodriguez C, et al.
Anaerobe, 17(1), 15-18 (2011)
A Felten et al.
Journal of clinical microbiology, 37(3), 729-733 (1999-02-13)
Lactobacilli recovered from the blood, cerebrospinal fluid, respiratory tract, and gut of 20 hospitalized immunocompromised septic patients were analyzed. Biochemical carbohydrate fermentation and total soluble cell protein profiles were used to identify the species. Hydrogen peroxide production was measured. Susceptibility...

Artigos

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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