T3405

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

Name: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride
Brand: SIGMA

chromogenic, tablet

Sinônimo(s):

4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

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Sobre este item

Fórmula empírica (Notação de Hill):

C₁₆H₂₀N₂ · 2HCl

Número CAS:

64285-73-0

Peso molecular:

313.27

NACRES:

NA.83

PubChem Substance ID:

24900140

UNSPSC Code:

12352204

EC Number:

264-769-6

MDL number:

MFCD00012961

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Nome do produto

3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet

InChI

1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

InChI key

NYNRGZULARUZCC-UHFFFAOYSA-N

SMILES string

Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

form

tablet

mp

≥300 °C

storage temp.

2-8°C

Quality Level

200

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Protein Biology

Proteins & Enzymes

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Este Item	693014	96738	SML3593
Sigma-Aldrich T3405 3,3′,5,5′-Tetramethylbenzidine dihydrochloride	Sigma-Aldrich 693014 (S)-H₈-BINAP	Sigma-Aldrich 96738 (R)-(+)-5,5′-Dichloro-2,2′-bis(diphenylphosphino)-6,6′-dimethoxy-1,1′-biphenyl	Sigma-Aldrich SML3593 Hexachlorophene
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
form tablet	form powder	form crystals	form powder
storage temp. 2-8°C	storage temp. -	storage temp. -	storage temp. 2-8°C
mp ≥300 °C	mp -	mp -	mp -

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm.

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the

anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera[1]
anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate[2]
in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients[3]

Classe de armazenamento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection.

Sandra Oloketuyi et al.

Biosensors, 11(12) (2021-12-24)

C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production, simple engineering, and the possibility to obtain higher

CD45 isoform expression is associated with different susceptibilities of human naive and effector CD4+ T cells to respond to IL-4

Stutz A, et al.

European Journal of Immunology, 35(2), 575-583 (2005)

Evaluation of antibody response to an adjuvanted hapten-protein vaccine as a potential inhibitor of sexual maturation for farmed Atlantic salmon.

Darcy S O Mora et al.

Fish & shellfish immunology, 71, 255-263 (2017-09-04)

An experimental contraceptive vaccine was evaluated in Atlantic salmon (Salmo salar). A peptide derived from the beta subunit of luteinizing hormone (LH) was conjugated to two different carrier proteins, bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH), and formulated

Reduced helminth burden increases allergen skin sensitization but not clinical allergy: a randomized, double-blind, placebo-controlled trial in Vietnam.

C Flohr et al.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 40(1), 131-142 (2009-09-18)

Observational evidence suggests that infection with helminths protects against allergic disease and allergen skin sensitization. It is postulated that such effects are mediated by helminth-induced cytokine responses, in particular IL-10. We tested this hypothesis in a rural area of central

A novel assay for the evaluation of the prooxidant-antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients

Alamdari DH, et al.

Clinical Biochemistry, 40(3-4), 248-254 (2007)

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Artigos

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Conteúdo relacionado

Data Sheet - T3405

Instructions

Product_Information_Sheet-T3405

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