Merck
All Photos(3)

T4661

Sigma-Aldrich

Trizma® base

≥99.9% (titration), crystalline

Synonym(s):
Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.25

Nível de qualidade

400

descrição

aminopeptidase substrate

teor

≥99.9% (titration)

forma

crystalline

perda

≤5% (loss on drying, 110 °C)

cor

white

pH

10.0-11.5

useful pH range

7-9

pKa (25 °C)

8.1

pb

219-220 °C/10 mmHg (lit.)

pf

167-172 °C (lit.)

solubilidade

water: soluble (400 g plus 600 mL of water)

λ

(290nm < 0.05)
in 5 mM Tris; DNA (40% (w/w))

application(s)

diagnostic assay manufacturing

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Descrição geral

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Aplicação

Tris base has been used:
  • As a component of lysis buffer for cell disruption
  • In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)
  • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
  • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Embalagem

100 g in poly bottle
1 kg in poly bottle
5 kg in poly drum
Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.

Nota de análise

Not pyrogen tested. Certificates of analysis are available with lot specific information.

Outras notas

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Informações legais

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service