Merck
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T6066

Sigma-Aldrich

Trizma® base

BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

Sinônimo(s):
Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
Fórmula linear:
NH2C(CH2OH)3
Número CAS:
Peso molecular:
121.14
Beilstein:
741883
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.25

grau

BioPerformance Certified

Nível de qualidade

400

Agency

USP/NF
meets EP testing specifications
meets USP testing specifications

descrição

aminopeptidase substrate

teor

≥99.9% (titration)

forma

crystalline powder

technique(s)

cell culture | mammalian: suitable

Impurezas

DNase, RNase, protease, none detected
Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested
≤0.2% water (Karl Fischer)

perda

≤0.5% (loss on drying, 110 °C)

pH

10.0-11.5

useful pH range

7-9

pKa (25 °C)

8.1

pb

219-220 °C/10 mmHg (lit.)

pf

167-172 °C (lit.)

solubilidade

water: soluble (666 mg/ml, yielding a clear colorless solution)

absorção

≤0.05 at 260 at 1 M
≤0.05 at 290 at 40%
≤0.06 at 280 at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Descrição geral

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Aplicação

Trizma has been used:
  • As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
  • As a component of pre-hybridization buffer in in situ hybridization
  • To suspend dried protein before SDS-PAGE protein characterization
  • As a component of lysis buffer to wash and lyse cells before immunoprecipitation
  • In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
  • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
  • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Embalagem

1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
T6066-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Outras notas

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.

Informações legais

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Gloves

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

Culture of human embryonic stem cells on human and mouse feeder cells.
Turksen, Kursad
Human Embryonic Stem Cell Protocols, 91-104 (2006)
Recurrent mutations in the MTOR regulator RRAGC in follicular lymphoma.
Peterson LF, et al.
Clinical Cancer Research, 22(21), 5383-5393 (2016)
Membrane proteins and proteomics: un amour impossible?
Santoni V, et al.
Electrophoresis, 21(6), 1054-1070 (2000)
Capillary electrochromatographic evaluation of vitamin active oil constituents: tocopherols and tocotrienols.
Abidi SL and Rennick KA
Journal of Chromatography A, 913(1-2), 379-386 (2001)
Recognition and strand displacement of DNA oligonucleotides by peptide nucleic acids (PNAs). High-performance ionexchange chromatographic analysis
Lesignoli F, et al.
Journal of Chromatography A, 922(1-2), 177-185 (2001)

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