Merck
Todas as fotos(3)

T7024

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, for molecular biology, ≥99% (GC)

Sinônimo(s):
TEMED, 1,2-Bis(dimethylamino)ethane, TMEDA
Fórmula linear:
(CH3)2NCH2CH2N(CH3)2
Número CAS:
Peso molecular:
116.20
Beilstein:
1732991
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.31

Nível de qualidade

200

grau

for molecular biology

linha de produto

BioReagent

teor

≥99% (GC)

Lim. expl.

9.08 %

índice de refração

n20/D 1.4179 (lit.)

pb

120-122 °C (lit.)

pf

−55 °C (lit.)

densidade

0.775 g/mL at 20 °C (lit.)

atividade externa

DNase, RNase, protease, none detected

SMILES string

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

InChI key

KWYHDKDOAIKMQN-UHFFFAOYSA-N

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Aplicação

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules.

Embalagem

25, 50, 100 mL in glass bottle

Quantidade

Suggested use is 35 μl for 50 ml acrylamide gel solution.

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Código de classe de armazenamento

3 - Flammable liquids

WGK

WGK 1

Ponto de fulgor (ºF)

61.7 °F - closed cup

Ponto de fulgor (ºC)

16.5 °C - closed cup

Equipamento de proteção individual

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificado de análise

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Certificado de origem

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Andrews, A. T.
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications, 21-24 (1986)
Sambrook, J. and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 5-5 (2001)
A V Vasiliskov et al.
BioTechniques, 27(3), 592-594 (1999-09-18)
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules
Arvind Chandrasekaran et al.
Lab on a chip, 19(17), 2786-2798 (2019-07-25)
To study respiratory diseases, in vitro airway epithelial models are commonly implemented by culturing airway cells on a porous surface at an air-liquid interface (ALI). However, these surfaces are often supraphysiologically stiff, which is known to affect the organization, maturation
Deepa Mohan et al.
Journal of chromatography. A, 979(1-2), 271-276 (2002-12-25)
The non-availability of commercial carrier ampholytes in the pH range greater than 11 has contributed to difficulties in focusing and resolving highly basic proteins/peptides using capillary isoelectric focusing (cIEF). Two different approaches, involving the use of N,N,N',N'-tetramethylethylenediamine (TEMED) and ampholyte

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