As mentioned on the Product Detail Page at the DESCRIPTION section, under 'Preparation Note' , this product is prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.
T8154
Azul de triptano
0.4%, liquid, sterile-filtered, suitable for cell culture
Sinônimo(s):
Blue cell viability dye
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R$ 88,00
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About This Item
R$ 88,00
Em estoqueDetalhes
Produtos recomendados
esterilidade
sterile-filtered
Nível de qualidade
Formulário
liquid
condição de armazenamento
dry at room temperature
concentração
0.4%
técnica(s)
cell culture | mammalian: suitable
tissue processing: suitable
aplicação(ões)
cell analysis
Condições de expedição
ambient
cadeia de caracteres SMILES
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
chave InChI
GLNADSQYFUSGOU-UHFFFAOYSA-J
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Descrição geral
Aplicação
- no ensaio de viabilidade celular para contar células viáveis e mortas[4]
- no ensaio de resgate para contar células viáveis no método de contagem de exclusão de[4]
- azul de triptano para determinar a curva de proliferação[5]
- como controle salino e na preparação de solução de lipopolissacarídeo (LPS) para confirmar o sucesso da infusão de LPS[6]
- no teste de exclusão do corante azul de tripano para determinar a viabilidade das células epiteliais gástricas (GEC)[7]
- para contar as células GEC usando um hemocitômetro[7]
Nota de preparo
geralmente comprado junto com este produto
produto comparável
recomendado
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Carc. 1B
Código de classe de armazenamento
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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is this solution dissolved in PBS?
1 answer-
Helpful?
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What is the stability of the product. How long we can use it as there is no expiry date mentioned in the COA
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Why is Trypan blue, Product T8154, used to determine cell viability?
1 answer-
Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.
Helpful?
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Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?
1 answer-
Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.
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Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?
1 answer-
Trypan blue will stain cells that have disrupted membranes. It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.
Helpful?
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Trypan blue, Product T8154, contains a precipitate. Is it still good to use?
1 answer-
The trypan blue solution is still good to use. It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.
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Why are there red fibers in Product T8154, Trypan blue solution?
1 answer-
A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality. The presence of fibers may be more pronounced following cold storage or shipping. This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.
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