Merck
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T8154

Sigma-Aldrich

Azul de triptano

0.4%, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
Blue cell viability dye
Empirical Formula (Hill Notation):
C34H24N6Na4O14S4
CAS Number:
Molecular Weight:
960.81
Beilstein:
4360496
Número MDL:
ID de substância PubChem:
NACRES:
NA.75

esterilidade

sterile-filtered

Nível de qualidade

forma

liquid

condição de armazenamento

dry at room temperature

concentração

0.4%

technique(s)

cell culture | mammalian: suitable
tissue processing: suitable

application(s)

cell analysis

enviado em

ambient

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

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This Item
T614630264393595
Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

T8154

Trypan Blue solution

Trypan Blue powder, BioReagent, suitable for cell culture

Sigma-Aldrich

T6146

Trypan Blue

Trypan Blue Dye content 60 %

Sigma-Aldrich

302643

Trypan Blue

Trypan Blue solution 0.4%, for microscopy

Sigma-Aldrich

93595

Trypan Blue solution

form

liquid

form

powder

form

powder

form

liquid

concentration

0.4%

concentration

-

concentration

-

concentration

0.4%

technique(s)

cell culture | mammalian: suitable, tissue processing: suitable

technique(s)

cell culture | mammalian: suitable, tissue processing: suitable

technique(s)

-

technique(s)

-

application(s)

cell analysis

application(s)

cell analysis

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

Descrição geral

O azul de triptano é um corante usado para distinguir entre células vivas e mortas. É uma mancha vital que não é absorvida por células viáveis saudáveis, mas que cora as células com uma membrana celular danificada. Com isso, apenas células mortas podem ser contadas. O método é às vezes referido como método de exclusão de corantes.
Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.

Aplicação

Trypan Blue solution has been used:
  • in cell viability assay to count viable and dead cells
  • in rescue assay to count viable cells
  • in trypan blue exclusion counting method to determine proliferation curve
  • as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
  • in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
  • to count GEC cells using a hemocytometer

Nota de preparo

Preparado em cloreto de sódio 0,81% e fosfato de potássio dibásico 0,06%.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Carc. 1B

Código de classe de armazenamento

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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