Todas as fotos(1)



Trypan Blue solution

0.4%, liquid, sterile-filtered, suitable for cell culture

Blue cell viability dye
Empirical Formula (Hill Notation):
Número CAS:
Peso molecular:
Número MDL:
ID de substância PubChem:

Nível de qualidade






condição de armazenamento

dry at room temperature




cell culture | mammalian: suitable
tissue processing: suitable


cell analysis

enviado em


SMILES string




InChI key


Procurando produtos similares? Visit Guia de comparação de produtos

Descrição geral

Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.


Use to detect dead and dying cells in cytotoxicity assays and for routine assessment of cell viability and proliferative assays.

Nota de preparo

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.


Health hazard

Palavra indicadora


Frases de perigo

Classificações de perigo

Carc. 1B

Código de classe de armazenamento

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects



Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

Mais documentos

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?

    Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.

  3. Why is Trypan blue, Product T8154, used to determine cell viability?

    Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.

  4. Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?

    Trypan blue will stain cells that have disrupted membranes.  It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.

  5. Trypan blue, Product T8154, contains a precipitate.  Is it still good to use?

    The trypan blue solution is still good to use.  It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Why are there red fibers in Product T8154, Trypan blue solution?

    A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality.  The presence of fibers may be more pronounced following cold storage or shipping.  This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

The sensitivity of 5-formylcytosine to doxorubicin regardless of DNA damage
Turkiye Entomoloji Dergisi (Turkish Journal of Entomology), 40 (2016)
Single cell isolation process with laser induced forward transfer
Yu Deng
Journal of Biological Engineering, 11(2) (2017)
Roya Khosravi-Far, Zahra Zakeri
Programmed Cell Death (2008)
Ulrich Putz et al.
Science signaling, 5(243), ra70-ra70 (2012-09-27)
Exosomes are microvesicles of endosomal origin that are secreted, and their contents (proteins, lipids, DNA, or microRNAs) can alter the physiological states of recipient cells. We demonstrated that phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor
S R Mishra et al.
Research in veterinary science, 108, 98-111 (2016-09-25)
The present study investigated the expression and localization of FGF and its functional receptors in the follicle of buffalo and the treatment of FGF2 on mRNA expression of CYP19A1 (aromatase), PCNA, and BAX (BCL-2 associated X protein) in cultured buffalo


Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.


Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Conteúdo relacionado

Preparation & Staining of 3D Printed Tissues & Scaffolds

Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica