Human transferrin has an approximate molecular weight range of 76-81 kDa. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/418/013/t8158pis.pdf
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| Tamanho da embalagem | SKU | Disponibilidade | Preço |
|---|---|---|---|
| 100 mg | Disponível para envio HOJEdeCajamar | R$ 1.031,00 | |
| 1 g | Verificar disponibilidade no carrinho | R$ 6.312,00 |
Sobre este item
General description
Application
- para incubar células do túbulo proximal humano (HKC) - 8 células juntamente com IgG para avaliar a especificidade da albumina[1]
- como um suplemento em meio contendo/sem soro para cultura de células de tireoide humanas
- como uma glicoproteína-alvo para desenvolver um método de hidrazinólise em gel
Biochem/physiol Actions
Preparation Note
Analysis Note
Other Notes
Disclaimer
1 of 1
Este Item | |||
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| technique(s) cell culture | mammalian: suitable | technique(s) immunoprecipitation (IP): suitable, tissue culture: suitable | technique(s) cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable |
| shipped in ambient | shipped in - | shipped in - | shipped in wet ice |
| solubility H2O: 50 mg/mL | solubility - | solubility - | solubility H2O: 50 mg/mL |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Classe de armazenamento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artigos
Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.
Conteúdo relacionado
Número de item de comércio global
| SKU | GTIN |
|---|---|
| T8158-1G | 04061837378935 |
| T8158-100MG | 04061835569670 |
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I want to know the Molecular Weight of this product.
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What is the correct method for storing item T8158, Human Transferrin, after reconstitution?
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After reconstitution, it is advisable to aliquot and freeze the product T8158, Human Transferrin at -20°C for long-term storage or add NaN₃ and refrigerate it at 4°C for short-term storage. Stock solutions can remain stable for up to 1 year at -20°C or for up to 2 weeks at 4°C.
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We were wondering how to store the dissolved transferrin. We have frozen it at -80. If we thaw it can we can freeze it again,
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Transferrin is fairly stable in solution. Non-sterile aqueous solutions may be stored at 4 °C for 5-10 days. Solutions may be filter-sterilized to extend solution stability. Various media supplements containing transferrin, such as ITS (Insulin/Transferrin/Selenium), are stable for up to 30 days at 4 °C and may be stored in frozen aliquots at -20 °C. for up to one year. Avoid repeated freeze/thaw cycles as this may contribute to precipitation occurring.
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How is this protein produced and what is the rest 2% impurity? Thanks!
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The preparation method is considered proprietary. The residual impurities have not been identified. This product is tested for endotoxin and iron. Please see the Sample Certificates of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T8158-BULKBCCK4250.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the carbohydrate content of human transferrin?
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We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.
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