Merck
All Photos(2)

T8768

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate

powder, ≥98%

Synonym(s):
TMB dihydrochloride
Linear Formula:
[-C6H2(CH3)2-4-NH2]2·2HCl·xH2O
CAS Number:
Molecular Weight:
313.27 (anhydrous basis)
Número MDL:
ID de substância PubChem:
NACRES:
NA.83

Nível de qualidade

200

teor

≥98%

forma

powder

solubilidade

acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy

temperatura de armazenamento

2-8°C

SMILES string

O.Cl.Cl.Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2

InChI key

KVCWTKDFVVSVSJ-UHFFFAOYSA-N

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Descrição geral

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase.[1] It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.[2]

Aplicação

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay[3] and reverse dot blot (RDB).[4]
Noncarcinogenic substitute for benzidine.

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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