Pular para o conteúdo
Merck

Pular para

T8787

Triton X-100

Molecular Biology

Triton™ X-100

Sinônimo(s):

t-Octilfenoxipolietoxietanol, Polietilenoglicol terc-octilfenil éter

Faça login para ver os preços organizacionais e de contrato.

Selecione um tamanho

Alterar visualização
Para você/SKUDisponibilidadePreço
50 mL

Disponível para envio HOJEdeCajamar

R$ 156,00
100 mL

Disponível para envio HOJEdeCajamar

R$ 223,00
250 mL

Disponível para envio HOJEdeCajamar

R$ 452,00

Sobre este item

Fórmula linear:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Número CAS:
UNSPSC Code:
12161900
NACRES:
NA.21
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
2315025

R$ 156,00


Disponível para envio HOJEDetalhes


Solicite um pedido personalizado
Assistência Técnica
Precisa de ajuda? Nossa equipe de cientistas experientes está à sua disposição.
Estamos aqui para ajudar


biological source

synthetic (organic)

Quality Segment

grade

Molecular Biology

description

non-ionic

form

liquid

mol wt

micellar avg mol wt 80,000, average mol wt 625

aggregation number

100-155

technique(s)

protein purification: suitable, protein quantification: suitable, western blot: suitable

impurities

<1.00% water (Karl Fischer)

pH

9.7

CMC

0.2-0.9 mM (20-25°C)

mp

6 °C

transition temp

cloud point 65 °C, pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

density

1.06 g/mL at 25 °C (lit.)

cation traces

Fe: <5 ppm, K: <0.05%, Na: <0.1%, heavy metals: <5 ppm

HLB

13.5

application(s)

hematology
histology

foreign activity

DNase and RNase, none detected

storage temp.

room temp

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

General description

Triton X-100 é um tensoativo e emulsificante não iônico comum que é usado com frequência em aplicações bioquímicas para solubilizar proteínas. É considerado um detergente comparativamente leve, não desnaturante, e foi relatado em muitas referências como um reagente rotineiramente adicionado. É utilizado para lisar células para extrair proteínas e organelas celulares. Também é capaz de permeabilizar membranas de células vivas para transfecção.

Application

O Triton X-100 foi usado:
  • Em imuno-histoquímica para corar retinas montadas em lâminas em camada plana[1]
  • Juntamente com PBS (solução salina tamponada com fosfato) gelado em suspensão de células para análise de DNA celular e ensaio de anexina V
  • Para permeabilizar células durante estudos microscópicos imunofluorescentes[2]
  • Como controle positivo no ensaio de LDH para determinar a integridade da membrana celular[3]
  • Para estimar a atividade da lipase no plasma pós-heparina usando o procedimento radioenzimático de Belfrage e Vaughan modificado
  • Para o preparo de extrato de proteínas de membrana externa
  • Como componente de tampão de extração, juntamente com tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35, para homogenização de células pulmonares de camundongos
  • No tratamento de cortes de tecido para marcação para imunofluorescência

Biochem/physiol Actions

Tensoativo não iônico amplamente utilizado para recuperar componentes de membrana em condições brandas não desnaturantes.

Features and Benefits

  • Ideal para aplicações sensíveis de pesquisa em biologia molecular
  • Testado para DNase e RNase
  • Detergente não iônico umectante
  • Agente umectante e emulsificante superior
  • Surfactante altamente versátil

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Comparar Itens Semelhantes

Ver Comparação Total

Mostrar diferenças

1 of 1

Este Item
X1001.08643X114
Triton&#8482; X-100 Molecular Biology

Sigma-Aldrich

T8787

Triton X-100

Triton&#8482; X-100 laboratory grade

Sigma-Aldrich

X100

Triton X-100

Triton&#8482; X-100 EMPROVE&#174; EXPERT Ph Eur

SAFC

1.08643

Triton X-100

Triton&#8482; X-114 laboratory grade

Sigma-Aldrich

X114

Triton X-114

technique(s)

protein purification: suitable, western blot: suitable, protein quantification: suitable

technique(s)

-

technique(s)

-

technique(s)

-

grade

Molecular Biology

grade

laboratory grade

grade

-

grade

laboratory grade

form

liquid

form

liquid

form

liquid

form

-

aggregation number

100-155

aggregation number

100-155

aggregation number

100-155

aggregation number

-

application(s)

hematology
histology

application(s)

advanced drug delivery

application(s)

-

application(s)

-

storage temp.

room temp

storage temp.

-

storage temp.

15-25°C

storage temp.

-


Still not finding the right product?

Explore all of our products under Triton X-100


Classe de armazenamento

10 - Combustible liquids

signalword

Danger

wgk

WGK 3

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2



Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

Se precisar de ajuda, entre em contato Atendimento ao cliente

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos



Questions

1–6 of 6 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the concentration of Triton X-1000 T8787?

    1 answer
    1. This product is considered a neat or pure liquid at 100% active ingredient. The maximum allowable water content is 1%. This value is lot specific and reported on the Certificate of Analysis.

      Helpful?

  4. Hello, I am wondering how could I find the purity of the Triton X-100 in your products. Your help and advice is appreciated. Kind regards, Soheil

    1 answer
    1. This product is 100 % active ingredient. The maximum allowable water content is 1 % for this product.

      Please see the link below to review a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T8787-VAR0000267901.pdf

      Helpful?

  5. Can pure Triton X-100 be stored at and remain stable at less than 4C?

    1 answer
    1. This product has been subjected, in the pure form, to temperatures at or near the pour point (1 °C) with no degradation. At lower temperatures, the material may become viscous and require gentle warming to ensure homogeneity. In dilute solutions, such as cell lysates or protein samples, Triton X-100 is an excellent freeze/thaw stabilizer.

      Helpful?

  6. What form is this in?

    1 answer
    1. This product is in liquid form at room temperature (melting point is 6 deg C) as listed in the "Properties" section of the product detail page.

      Helpful?

Reviews

Active Filters

  1. 1 Ratings-only review