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T8787

Sigma-Aldrich

Triton X-100

for molecular biology

Sinônimo(s):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Fórmula linear:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Número CAS:
Beilstein:
2315025
Número MDL:
ID de substância PubChem:
NACRES:
NA.21

Nível de qualidade

200

grau

for molecular biology

descrição

non-ionic

forma

liquid

peso molecular

micellar avg mol wt 80,000
average mol wt 625

número de agregação

100-155

technique(s)

protein purification: suitable
protein quantification: suitable
western blot: suitable

pH

9.7

CMC

0.2-0.9 mM (20-25°C)

pf

6 °C

temperatura de transição

cloud point 65 °C
pour point ~7 °C

solubilidade

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

densidade

1.06 g/mL at 25 °C (lit.)

HLB

13.5

application(s)

diagnostic assay manufacturing
hematology
histology

atividade externa

DNase and RNase, none detected

temperatura de armazenamento

room temp

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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Descrição geral

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Aplicação

Triton X-100 has been used:
  • In immunohistochemistry for staining the Flat-mount retinas
  • Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
  • To permeabilise cells during Immunofluorescent microscopic studies
  • As a positive control in LDH assay to determine the cell membrane integrity
  • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
  • For the preparation of outer membrane protein exctract
  • As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells
  • In the treatment of tissue sections for Immunofluorescence labeling

Embalagem

50, 100, 250 mL in poly bottle

Ações bioquímicas/fisiológicas

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Informações legais

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

WGK

WGK 3

Ponto de fulgor (ºF)

483.8 °F

Ponto de fulgor (ºC)

251 °C

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

M A Ferguson et al.
Journal of applied physiology (Bethesda, Md. : 1985), 85(3), 1169-1174 (1998-09-08)
The purpose of this study was to determine the threshold of exercise energy expenditure necessary to change blood lipid and lipoprotein concentrations and lipoprotein lipase activity (LPLA) in healthy, trained men. On different days, 11 men (age, 26.7 +/- 6.1
Jean-Sébastien Joyal et al.
Blood, 117(22), 6024-6035 (2011-03-01)
The failure of blood vessels to revascularize ischemic neural tissue represents a significant challenge for vascular biology. Examples include proliferative retinopathies (PRs) such as retinopathy of prematurity and proliferative diabetic retinopathy, which are the leading causes of blindness in children
Effects of four different single exercise sessions on lipids, lipoproteins, and lipoprotein lipase.
Ferguson M A, et al.
Journal of Applied Physics, 85(3), 1169-1174 (1998)
Eric Soupene et al.
Experimental biology and medicine (Maywood, N.J.), 233(5), 507-521 (2008-04-01)
Acyl-CoA synthetase enzymes are essential for de novo lipid synthesis, fatty acid catabolism, and remodeling of membranes. Activation of fatty acids requires a two-step reaction catalyzed by these enzymes. In the first step, an acyl-AMP intermediate is formed from ATP.
Ki-Bum Kim et al.
Methods in molecular biology (Clifton, N.J.), 424, 413-422 (2008-03-29)
Because lipid rafts are plasma membrane platforms mediating various cellular events such as in signal transduction, immunological response, pathogen invasion, and neurodegenerative diseases, protein identification in the rafts could provide important information to study their function. Here, we present an

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