T9026

Monoclonal Anti-α-Tubulin antibody produced in mouse

clone DM1A, ascites fluid

• Synonym(s): Monoclonal Anti-α-Tubulin
• NACRES: NA.41

Properties

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: ascites fluid
• antibody product type: primary antibodies
• clone: DM1A, monoclonal
• peso molecular: antigen ~50 kDa
• contém: 15 mM sodium azide
• species reactivity: yeast, mouse, amphibian, human, rat, chicken, fungi, bovine
• validação aprimorada: independent ( antibodies); Learn more about Antibody Enhanced Validation
• technique(s): indirect immunofluorescence: 1:500 using cultured chicken fibroblasts; western blot: 1:500 using human or chicken fibroblasts
• Isotipo: IgG1
• nº de adesão UniProt: P68366
• application(s): research pathology
• enviado em: dry ice
• temperatura de armazenamento: −20°C
• Gene Information: human ... TUBA4A(7277) ; mouse ... Tuba1a(22142) ; rat ... Tuba1a(64158)

Description

Descrição geral

α-tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC. Anti-α-Tubulin FITC antibody, Mouse monoclonal specifically recognizes an epitope in the carboxy terminal part of α-tubulin. It localizes α-tubulin in human, monkey, bovine, chicken, goat, murine, rat, gerbil, hamster, rat kangaroo, amphibia, sea urchin, trypanosome, yeast, fungi and tobacco.

Especificidade

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.

Imunogênio

microtubules from chicken embryo brain.

Aplicação

Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in western blot analysis and protein expression analysis.
Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in the detection of α-Tubulin:

• in human osteosarcoma and in breast cancer cell lines by western blotting
• in HeLa cells by immunofluorescence microscopy
• by immunostaining
• immunohistochemical detection in Xenopus embryos

Ações bioquímicas/fisiológicas

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α/β-Tubulin is the major building block of microtubules. These intracellular, hollow, cylindrical, filamentous structures are present in virtually all eukaryotic cells. Self-assembly of α/β-tubulin leads to polar microtubular structures built from linearly arranged strings of alternatively α- and β-tubulin pairs pointing in the same direction. Microtubules function as structural and mobile elements in mitosis, intracellular transport, ciliary flagellar motility and generation and maintenance of cell shape. α/β-Tubulin and γ-tubulin are members of the tubulin superfamily of proteins. α/β-Tubulin is a heterodimer which consists of one α-tubulin chain and one β-tubulin chain; each subunit has a molecular weight of 55 kDa and they share considerable homolog.

Armazenamento e estabilidade

For continuous use, store at 2-8 °C for up to one month.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Código de classe de armazenamento: 10 - Combustible liquids
• WGK: WGK 3
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable