U5378

Urea

powder, BioReagent, for molecular biology, suitable for cell culture

• Sinônimo(s): Carbonyldiamide, Carbamide
• Fórmula linear: NH₂CONH₂
• Número CAS: 57-13-6
• Peso molecular: 60.06
• Beilstein: 635724
• Número EC: 200-315-5
• Número MDL: MFCD00008022
• ID de substância PubChem: 24900656
• NACRES: NA.31

Propriedades

• Nível de qualidade: 200
• grau: for molecular biology
• linha de produto: BioReagent
• teor: ≥98%
• forma: powder
• technique(s): cell culture | mammalian: suitable
• pf: 132-135 °C (lit.)
• solubilidade: H₂O: soluble 480 mg/mL, clear, colorless (8M)
• densidade: 1.335 g/mL at 25 °C (lit.)
• traços de ânion: chloride (Cl^-): ≤5 ppm
• traços de cátion: Cu: ≤1 ppm; Fe: ≤5 ppm; Pb: ≤2 ppm
• absorção: ≤0.06 at 260 at 6 M; ≤0.06 at 280 at 6 M
• atividade externa: DNase, NICKase, RNase and protease, none detected
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• Gene Information: human ... CA1(759) , CA2(760) ; rat ... Ppm1a(24666)

Descrição

Aplicação

• Urea has been used in protein resuspension buffer and to denature proteins.
• It has been used for the isolation of proteins via urea-containing polyacrylamide gels.
• It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Embalagem

1 kg in poly bottle

100, 500 g in poly bottle

5 kg in poly drum

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• WGK: WGK 1
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)