Merck
Todas as fotos(4)

U5378

Sigma-Aldrich

Urea

powder, BioReagent, for molecular biology, suitable for cell culture

Sinônimo(s):
Carbonyldiamide, Carbamide
Fórmula linear:
NH2CONH2
Número CAS:
Peso molecular:
60.06
Beilstein:
635724
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.31

Nível de qualidade

200

grau

for molecular biology

linha de produto

BioReagent

teor

≥98%

forma

powder

technique(s)

cell culture | mammalian: suitable

pf

132-135 °C (lit.)

solubilidade

H2O: soluble 480 mg/mL, clear, colorless (8M)

densidade

1.335 g/mL at 25 °C (lit.)

traços de ânion

chloride (Cl-): ≤5 ppm

traços de cátion

Cu: ≤1 ppm
Fe: ≤5 ppm
Pb: ≤2 ppm

absorção

≤0.06 at 260 at 6 M
≤0.06 at 280 at 6 M

atividade externa

DNase, NICKase, RNase and protease, none detected

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

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Aplicação

  • Urea has been used in protein resuspension buffer and to denature proteins.
  • It has been used for the isolation of proteins via urea-containing polyacrylamide gels.
  • It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Embalagem

1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

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Certificado de origem

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Labeling and identification of direct kinase substrates.
Carlson SM and White FM
Science Signaling, 5, pl3-pl3 (2012)
Construction and transfection of a ribozyme targeting human caspase-3.
Cronin D et al.
Anticancer Research, 24, 425-425 (2004)
Structure and properties of native and unfolded lysing enzyme from T. harzianum: Chemical and pH denaturation.
Bey H et al.
International Journal of Biological Macromolecules, 92, 860-860 (2016)
Ziyi Yin et al.
Autophagy, 15(7), 1234-1257 (2019-02-20)
Macroautophagy/autophagy is critical for normal appressorium formation and pathogenicity of the rice blast fungus Magnaporthe oryzae, but the molecular base of autophagy linked to pathogenicity remains elusive in this or other pathogenic fungi. We found that MoHat1, a histone acetyltransferase
Gurudayal et al.
Nano letters, 15(6), 3833-3839 (2015-05-06)
Photoelectrochemical water splitting half reactions on semiconducting photoelectrodes have received much attention but efficient overall water splitting driven by a single photoelectrode has remained elusive due to stringent electronic and thermodynamic property requirements. Utilizing a tandem configuration wherein the total

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