X0629

Sigma-Aldrich

Anti-XRCC1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinônimo(s):
Anti-X-ray repair complementing defective repair in Chinese hamster cells 1
Número MDL:

fonte biológica

rabbit

Nível de qualidade

200

forma do anticorpo

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

antigen 85 kDa

species reactivity

human

concentração

~1 mg/mL

aplicação(ões)

indirect immunofluorescence: 5-10 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton
western blot: 0.5-1 μg/mL using A431 cell lysates

conjugado

unconjugated

nº de adesão UniProt

enviado em

dry ice

temperatura de armazenamento

−20°C

Gene Information

human ... XRCC1(7515)
mouse ... Xrcc1(22594)
rat ... Xrcc1(84495)

Descrição geral

X-ray repair cross-complementing group 1 (XRCC1) is a testis enriched protein. It is considered as a DNA repair gene.

Imunogênio

synthetic peptide corresponding to amino acids 615-633 of human XRCC1, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is identical in mouse and rat.

Aplicação

Anti-XRCC1 antibody produced in rabbit has been used in:
  • immunoblotting
  • immunofluorescence
  • indirect immunofluorescence and telomere FISH (TEL-FISH)
  • protein detection
  • western analysis

Ações bioquímicas/fisiológicas

X-ray repair cross-complementing group 1 (XRCC1) is a molecular scaffold protein that coordinates the assembly of repair complexes at damaged sites. XRCC has been shown to physically interact with several enzymes known to be involved in the repair of SSBs, including DNA ligase IIIa, DNA polymerase b, APE1, polynucleotide kinase (PNK), poly (ADP-ribose), polymerases 1 and 2 (PARP-1 and 2) and others. It participates in the DNA base excision repair (BER) pathway.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Alemanha

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificado de análise

Certificado de origem

XRCC1 deficiency correlates with increased DNA damage and male infertility
Singh V, et al.
Mutation Research. Genetic Toxicology and Environmental Mutagenesis, 839, 1-8 (2019)
XRCC1 deficiency correlates with increased DNA damage and male infertility
Singh V, et al.
Mutation Research. Genetic Toxicology and Environmental Mutagenesis, 839 (2019)
X-ray repair cross complementing protein 1 in base excision repair
Hanssen-Bauer A, et al.
International Journal of Molecular Sciences, 13(12), 17210-17229 (2012)
Characterization of oxidative guanine damage and repair in mammalian telomeres
Wang Z, et al.
PLoS Genetics, 6(5), e1000951-e1000951 (2010)
XRCC1 polymorphism and overall survival in ovarian cancer patients treated with platinum-based chemotherapy: A systematic review and MOOSE-compliant meta-analysis
Zhang Z, et al.
Medicine, 97(45) (2018)

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