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" no prazo de Papéis
Kristina Xiao Liang et al.
EMBO molecular medicine, 12(10), e12146-e12146 (2020-08-26)
Mutations in POLG disrupt mtDNA replication and cause devastating diseases often with neurological phenotypes. Defining disease mechanisms has been hampered by limited access to human tissues, particularly neurons. Using patient cells carrying POLG mutations, we generated iPSCs and then neural
Giulia Salvadori et al.
Cell metabolism, 33(11), 2247-2259 (2021-11-04)
Metastatic tumors remain lethal due to primary/acquired resistance to therapy or cancer stem cell (CSC)-mediated repopulation. We show that a fasting-mimicking diet (FMD) activates starvation escape pathways in triple-negative breast cancer (TNBC) cells, which can be identified and targeted by
Yingjie Liu et al.
Nature communications, 12(1), 5232-5232 (2021-09-04)
Disseminated tumor cells often fall into a long term of dormant stage, characterized by decreased proliferation but sustained survival, in distant organs before awakening for metastatic growth. However, the regulatory mechanism of metastatic dormancy and awakening is largely unknown. Here
Kimberly M Stauffer et al.
Cancer medicine, 10(21), 7692-7711 (2021-09-29)
Cancer resequencing studies have revealed epigenetic enzymes as common targets for recurrent mutations. The monomethyltransferase MLL3 is among the most recurrently mutated enzymes in ER+ breast cancer. The H3K4me1 marks created by MLL3 can define enhancers. In ER+ breast cancer, ERα genome-binding
Uma S Kamaraj et al.
Cell systems, 11(5), 509-522 (2020-10-11)
The need to derive and culture diverse cell or tissue types in vitro has prompted investigations on how changes in culture conditions affect cell states. However, the identification of the optimal conditions (e.g., signaling molecules and growth factors) required to maintain
Helen Zhao et al.
Frontiers in neuroscience, 14, 607711-607711 (2021-02-02)
Chronic mountain sickness (CMS) is a disease that potentially threatens a large segment of high-altitude populations during extended living at altitudes above 2,500 m. Patients with CMS suffer from severe hypoxemia, excessive erythrocytosis and neurologic deficits. The cellular mechanisms underlying
Shawna L McMillin et al.
International journal of molecular sciences, 22(9) (2021-06-03)
Denervation rapidly induces insulin resistance (i.e., impairments in insulin-stimulated glucose uptake and signaling proteins) in skeletal muscle. Surprisingly, whether this metabolic derangement is long-lasting is presently not clear. The main goal of this study was to determine if insulin resistance
Owen G James et al.
Developmental cell, 56(9), 1346-1358 (2021-05-05)
Myelination is essential for central nervous system (CNS) formation, health, and function. Emerging evidence of oligodendrocyte heterogeneity in health and disease and divergent CNS gene expression profiles between mice and humans supports the development of experimentally tractable human myelination systems.
Jean Berthelet et al.
Science advances, 7(28) (2021-07-09)
Intratumoral heterogeneity is a driver of breast cancer progression, but the nature of the clonal interactive network involved in this process remains unclear. Here, we optimized the use of optical barcoding to visualize and characterize 31 cancer subclones in vivo.
Thomas H Ambrosi et al.
eLife, 10 (2021-07-20)
Skeletal stem and progenitor cell populations are crucial for bone physiology. Characterization of these cell types remains restricted to heterogenous bulk populations with limited information on whether they are unique or overlap with previously characterized cell types. Here we show
Marco Tozzi et al.
Acta physiologica (Oxford, England), 228(2), e13360-e13360 (2019-08-11)
Extracellular ATP signalling is involved in many physiological and pathophysiological processes in several tissues, including adipose tissue. Adipocytes have crucial functions in lipid and glucose metabolism and they express purinergic receptors. However, the sources of extracellular ATP in adipose tissue
The insulin signaling system.
M F White et al.
The Journal of biological chemistry, 269(1), 1-4 (1994-01-07)
Hayma Hammoud et al.
Acta physiologica (Oxford, England), 232(2), e13623-e13623 (2021-02-10)
We examined if tonic γ-aminobutyric acid (GABA)-activated currents in primary hippocampal neurons were modulated by insulin in wild-type and tg-APPSwe mice, an Alzheimer's disease (AD) model. GABA-activated currents were recorded in dentate gyrus (DG) granule cells and CA3 pyramidal neurons
Linda Jacobsen et al.
BioTechniques, 47(1), 617-624 (2009-07-15)
Transfection is used to introduce a gene of interest into a cell. To interpret the downstream results, understanding which effects are the true biological responses to the gene and which, if any, are off-target effects can be difficult. In order
Marie S Isidor et al.
Adipocyte, 5(2), 175-185 (2016-07-08)
Brown adipose tissue is a promising therapeutic target for opposing obesity, glucose intolerance and insulin resistance. The ability to modulate gene expression in mature brown adipocytes is important to understand brown adipocyte function and delineate novel regulatory mechanisms of non-shivering
Rafael Contreras-Montoya et al.
ACS applied materials & interfaces, 13(10), 11672-11682 (2021-03-05)
Protein therapeutics have a major role in medicine in that they are used to treat diverse pathologies. Their three-dimensional structures not only offer higher specificity and lower toxicity than small organic compounds but also make them less stable, limiting their
E A Gorbunov et al.
Nutrition & diabetes, 5, e169-e169 (2015-07-07)
It has been previously shown that Subetta (a drug containing released-active forms of antibodies to the insulin receptor β-subunit and antibodies to endothelial nitric oxide synthase) stimulated insulin-induced adiponectin production by mature human adipocytes in the absence of insulin. Therefore
Renata M Martin et al.
Nature communications, 11(1), 2713-2713 (2020-06-03)
Despite their rapidly-expanding therapeutic potential, human pluripotent stem cell (hPSC)-derived cell therapies continue to have serious safety risks. Transplantation of hPSC-derived cell populations into preclinical models has generated teratomas (tumors arising from undifferentiated hPSCs), unwanted tissues, and other types of
Karl J Wahlin et al.
Frontiers in cell and developmental biology, 9, 764725-764725 (2021-12-07)
Human pluripotent stem cells (PSCs) represent a powerful tool to investigate human eye development and disease. When grown in 3D, they can self-assemble into laminar organized retinas; however, variation in the size, shape and composition of individual organoids exists. Neither
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