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Palavra-chave:'56197'
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56197

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Switching on luminescence in nucleotide/lanthanide coordination nanoparticles via synergistic interactions with a cofactor ligand.
Carole Aimé et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 16(12), 3604-3607 (2010-03-02)
Y Kong et al.
Rapid communications in mass spectrometry : RCM, 15(1), 57-64 (2001-01-03)
The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the bases of A
K Tang et al.
Nucleic acids research, 23(16), 3126-3131 (1995-08-25)
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation
Accurate characterization of carcinogenic DNA adducts using MALDI tandem time-of-flight mass spectrometry.
Barnes C A and Chiu N H
International Journal of Mass Spectrometry, 279(2-3), 170-175 (2009)
B A Bruenner et al.
Rapid communications in mass spectrometry : RCM, 10(14), 1797-1801 (1996-01-01)
Quantitative aspects of oligonucleotide analysis by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry remain largely unexplored relative to the efforts that have been devoted to quantitative peptide and protein analysis. The successful quantitation of these other biopolymers coupled with the potential
Indranil Sinha et al.
Angewandte Chemie (International ed. in English), 54(12), 3603-3606 (2015-01-30)
The first parallel-stranded DNA duplex with Hoogsteen base pairing that readily incorporates an Ag(+) ion into an internal mispair to form a metal-mediated base pair has been created. Towards this end, the highly stabilizing 6 FP-Ag(+)-6 FP base pair comprising the artificial
Gas-phase diagnostics and LIF-imaging of 3-hydroxypicolinic acid MALDI-matrix plumes
Puretzky AA and Geohegan DB
Chemical Physics Letters, 286(5-6), 425-432 (1998)
A V Streletskiĭ et al.
Bioorganicheskaia khimiia, 31(2), 151-158 (2005-05-14)
MALDI mass spectrometry (MS) of 14- to 42-mer homogeneous oligonucleotides and their mixtures was carried out using a Vision 2000 instrument (Thermo BioAnalysis, Finnigan, United States). Conditions for the determination of oligonucleotide molecular masses were optimized by applying various matrices
P Juhasz et al.
Carbohydrate research, 270(2), 131-147 (1995-04-30)
Molecular weights of heparin-derived oligosaccharides ranging from disaccharides to hexadecasaccharides have been determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. While these compounds ionize poorly or not at all when used as such, a strong signal can be obtained
K Tang et al.
Rapid communications in mass spectrometry : RCM, 8(9), 673-677 (1994-09-01)
We found that picolinic acid is a very good matrix for matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry of oligonucleotides, proteins and tRNA. Among the oligonucleotides, picolinic acid was shown to be effective for homo-oligonucleotides, d(G)40 and d(C)60, and for
Chih-Chi Chu et al.
Chembiochem : a European journal of chemical biology, 15(13), 1905-1910 (2014-07-25)
We report DNA catalysts (deoxyribozymes) that join tyrosine-containing peptides to RNA and DNA in one step and without requiring protecting groups on either the peptide or the nucleic acid. Our previous efforts towards this goal required tethering the peptide to
E Nordhoff et al.
Nucleic acids research, 22(13), 2460-2465 (1994-07-11)
UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a
J M Koomen et al.
Journal of mass spectrometry : JMS, 35(8), 1025-1034 (2000-09-06)
The changes in the ion signals in the isotope cluster, mass resolution, signal-to-noise ratio and mass accuracy for matrix-assisted laser desorption/ionization (MALDI) of DNA oligonucleotides (dGGATC, dCAGCt, and dAACCGTT) and their fragment ions were evaluated, and these data were compared
M S Abu-Bakr
Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986), 58(3), 231-236 (1993-01-01)
The complexation equilibria of iron(III) with 3-hydroxypicolinic acid (hypa) have been studied spectrophotometrically in 40% (v/v) ethanol-water medium at 25 degrees C and an ionic strength of 0.1 M NaClO4. The equilibria occurring in solution were established and the basic
K Curry et al.
Neuroscience letters, 66(1), 101-105 (1986-05-06)
The actions of acridinic acid (2,3-quinoline dicarboxylic acid), a new derivative of quinolinic acid, as an antagonist of amino acid-induced excitations are described. Acridinate, like kynurenate, in the cerebral cortex reduced the effects of all amino acids equally, but in
Jesse J Chen et al.
PloS one, 4(3), e4949-e4949 (2009-03-24)
Glycerol nucleic acid (GNA) has an acyclic phosphoglycerol backbone repeat-unit, but forms stable duplexes based on Watson-Crick base-pairing. Because of its structural simplicity, GNA is of particular interest with respect to the possibility of evolving functional polymers by in vitro
Wen-Hua Chiou et al.
The Journal of organic chemistry, 75(5), 1748-1751 (2010-02-09)
Facile syntheses of enantiopure trans- and cis-3-hydroxypiperidine derivatives and 3-hydroxypipecolic acids are reported, featuring Rh-catalyzed cyclohydrocarbonylation through common intermediates. A diaxial conformation in a 2,3-disubstituted N-Boc-piperidinyl structure is revealed by an X-ray crystallographic analysis.
Jennifer A Jacobsen et al.
Journal of medicinal chemistry, 54(2), 591-602 (2010-12-30)
Fragment-based lead design (FBLD) has been used to identify new metal-binding groups for metalloenzyme inhibitors. When screened at 1 mM, a chelator fragment library (CFL-1.1) of 96 compounds produced hit rates ranging from 29% to 43% for five matrix metalloproteases
Isolation and characterization of quinoline-degrading bacteria from subsurface sediments.
Applied and Environmental Microbiology, 55 (4), 1029-1032 (1989)
J Bai et al.
Rapid communications in mass spectrometry : RCM, 8(9), 687-691 (1994-09-01)
Matrix-assisted laser desorption/ionization using a 3-hydroxypicolinic acid matrix from an active Nafion substrate has been used for detection of restriction enzyme-digested double-stranded plasmid DNA using time-of-flight mass spectrometry. DNA strands of up to 267 base pairs were detected with minimal
Y Dai et al.
Rapid communications in mass spectrometry : RCM, 10(14), 1792-1796 (1996-01-01)
A method for accurate mass measurement of oligonucleotides up to a DNA 35-mer based on matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is described. In this method, a time-lag focusing time-of-flight mass spectrometer is used to achieve high mass resolution to
Li-Hua Zhou et al.
Rapid communications in mass spectrometry : RCM, 23(15), 2264-2272 (2009-06-25)
Application of matrix-assisted laser-desorption/ionization mass spectrometry (MALDI MS) to analysis and characterization of phosphopeptides in peptide mixtures may have a limitation, because of the lower ionizing efficiency of phosphopeptides than nonphosphorylated peptides in MALDI MS. In this work, a binary
H Suzuki et al.
Analytical chemistry, 69(22), 4554-4559 (1998-01-07)
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), has been used for structure characterization of 1-aminopyrene-3,6,8-trisulfonate (APTS)-derivatized oligosaccharides previously separated by capillary electrophoresis (CE). The resolved components were first isolated by employing an automated high-resolution fraction collector. Using on-probe sample
Ningning Liang et al.
The Journal of organic chemistry, 70(24), 10182-10185 (2005-11-19)
[reaction: see text] 3-Hydroxypipecolic acid, a nonproteinogenic cyclic alpha-amino acid, is a common structural moiety found in a large number of natural and synthetic compounds of medicinal significance. Utilizing D-serine as a chiral template, the present research describes efficient and
Maria Heinrich et al.
European journal of mass spectrometry (Chichester, England), 14(6), 401-409 (2009-01-13)
Six different glycosyl esters of nucleoside pyrophosphates (monosaccharide nucleotides) were analyzed by means of matrix-assisted laser desorption/ionisation reflectron time-of-flight mass spectrometry (MALDI/RToF MS) in the negative ion mode. Several matrices were evaluated and 3-hydroxypicolinic acid as well as alpha-cyano-4- hydroxycinnamic
Karel Waisser et al.
Archiv der Pharmazie, 336(6-7), 322-335 (2003-09-04)
A set of 40 derivatives of 3-hydroxypicolinic acid and 2-sulfanylbenzoic acid, isosteric to salicylanilides was synthesized. The compounds were evaluated for in vitro activity against Mycobacterium tuberculosis, Mycobacterium kansasii and Mycobacterium avium, Candida albicans, Candida tropicalis, Candida krusei, Candida glabrata
Ewa Radzikowska et al.
Organic & biomolecular chemistry, 13(1), 269-276 (2014-11-05)
Chimeric oligonucleotides containing phosphodiester and phosphorothioate linkages have been obtained using the solid phase synthesis. The oligonucleotide parts possessing natural internucleotide phosphate bonds were assembled using commercially available nucleoside 3'-O-(2-cyanoethyl-N,N-diisopropylamino)phosphoramidites 7 whereas the phosphorothioate segment was built using nucleoside 3'-O-(2-thio-1,3,2-oxathiaphospholanes)
Seth S Bradford et al.
ChemMedChem, 9(6), 1275-1285 (2014-04-24)
The complex Cu-GGHYrFK-amide (1-Cu) was previously reported as a novel metallotherapeutic that catalytically inactivates stem loop IIb (SLIIb) of the hepatitis C virus (HCV) internal ribosomal entry site (IRES) RNA and demonstrates significant antiviral activity in a cellular HCV replicon assay. Herein
Giedrius Sasnauskas et al.
Nucleic acids research, 36(12), 3969-3977 (2008-06-03)
Restriction endonucleases catalyse DNA cleavage at specific sites. The BfiI endonuclease cuts DNA to give staggered ends with 1-nt 3'-extensions. We show here that BfiI can also fill in the staggered ends: while cleaving DNA, it can add a 2'-deoxynucleoside
M S Shchepinov et al.
Nucleic acids research, 29(18), 3864-3872 (2001-09-15)
Chemical and enzymatic approaches were used to produce polynucleotide fragments containing acid-labile internucleotide P3'-N5' phosphoramidate bonds, either in a surface-bound form or in solution. The primer extension reaction utilizing 5'-amino-5'-deoxynucleoside 5'-triphosphates generates polynucleotides that can be fragmented into short, easy-to-analyze
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