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S Ishwarya et al.
Preparative biochemistry & biotechnology, 43(1), 95-107 (2012-12-12)
A serine protease was purified 6.9-fold from the leaves of Thespesia populnea using ammonium sulfate fractionation followed by CM-cellulose and Sephadex G-100 chromatography. The purified enzyme was named populnein and was characterized. It was made up of a single polypeptide
Xin Wang et al.
ACS nano, 8(12), 12151-12166 (2014-12-02)
Chemoresistance is a primary cause of treatment failure in cancer and a common property of tumor-initiating cancer stem cells. Overcoming mechanisms of chemoresistance, particularly in cancer stem cells, can markedly enhance cancer therapy and prevent recurrence and metastasis. This study
Shiwani Shukla et al.
Archiv der Pharmazie, 347(7), 486-495 (2014-05-02)
A series of 4,6-diaryl/heteroarylpyrimidones was synthesized employing silica-supported fluoroboric acid under solvent-free conditions in a microwave reactor. The catalytic influence of HBF4-SiO2 was investigated in detail to optimize the reaction conditions. The synthesized compounds were evaluated for in vitro xanthine
Urh Černigoj et al.
Journal of chromatography. A, 1281, 87-93 (2013-02-13)
To exploit different chromatographic modes for efficient plasmid DNA (pDNA) purification a novel monolithic chromatographic support bearing multimodal histamine (HISA) groups was developed and characterized. Electrostatic charge of HISA groups depends on the pH of the mobile phase, being neutral
Roni Yosipovich et al.
Vaccine, 33(3), 472-478 (2014-12-03)
In the first 10-14 days of a chick's life, protection is conferred by maternal antibodies. Further broiler protection is achieved by active vaccination. However, the high level of maternal antibodies interferes with the induction of an effective immune response by
Okhil K Nag et al.
Journal of pharmaceutical sciences, 104(1), 114-123 (2014-11-14)
We report the synthesis of an acyl-anchored superhydrophilic polymer (SHP) for external surface modification of liposome surface. N¹-(2-aminoethyl)-N⁴-hexadecyl-2-tetradecylsuccinamide conjugated with SHP (HDAS-SHP) was synthesized and used for modifying the liposome surface. Unlike polyethylene glycol (PEG)-phospholipids, which are commonly used for
Thiago S Frauches et al.
Toxicon : official journal of the International Society on Toxinology, 71, 49-56 (2013-06-05)
Neutralizing monoclonal antibodies against three major toxic components of Bothrops atrox venom were produced and tested. The mAbs against phospholipase A2, hemorrhagic metalloprotease, and thrombin-like enzymes were produced in large amounts and purified with caprylic acid followed by ammonium sulfate
Aubérie Parent et al.
Nature communications, 6, 5686-5686 (2015-01-20)
Friedreich's ataxia is a severe neurodegenerative disease caused by the decreased expression of frataxin, a mitochondrial protein that stimulates iron-sulfur (Fe-S) cluster biogenesis. In mammals, the primary steps of Fe-S cluster assembly are performed by the NFS1-ISD11-ISCU complex via the
Sruthi Purushothaman et al.
Journal of proteomics, 112, 113-124 (2014-09-03)
The extensive arm regeneration of brittle stars following amputation is becoming increasingly recognized as a model system for understanding cellular differentiation and regeneration in a whole animal context. In this study we have used the emerging brittle star model Amphiura
Kazunori Sugiura et al.
Biochemical and biophysical research communications, 457(3), 242-248 (2015-01-17)
Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004))
Hiroko Kobayashi et al.
Biological & pharmaceutical bulletin, 37(11), 1843-1847 (2014-11-05)
Here, we determined the sequence of a cDNA encoding a guanylic acid-specific ribonuclease (RNase He1) from Hericium erinaceus that exhibits high sequence identity (59%) with RNase Po1, an enzyme with anti-cancer activity and which is found in Pleurotus ostreatus. RNase
Silvia Llopis et al.
PloS one, 9(5), e98094-e98094 (2014-06-01)
Saccharomyces cerevisiae plays a beneficial role in health because of its intrinsic nutritional value and bio-functional properties, which is why it is also used as a dietary supplement. However, the perception that S. cerevisiae is harmless has changed due to
Małgorzata Anita Bryszewska et al.
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 29, 91-98 (2014-12-04)
In this study, we investigate the type and quantity of selenium compounds in fish and marine organisms, using ion-pair reversed phase LC–ICP-MS, developed and applied for the analysis of Atlantic cod, Atlantic salmon, Greenland halibut, Atlantic herring, blue mussel, common
Naozumi Ohnishi et al.
Chemical & pharmaceutical bulletin, 62(6), 538-544 (2014-06-03)
A novel in vitro release test methodology for a liposome formulation was developed using a column-switching high-performance liquid chromatography (HPLC) system. Doxorubicin (DXR) liposome formulations were used as a model. A DXR liposome formulation was dispersed into a release medium
Ryo Nasuno et al.
PloS one, 9(11), e113788-e113788 (2014-11-26)
The budding yeast Saccharomyces cerevisiae possesses various defense mechanisms against environmental stresses that generate reactive oxygen species, leading to growth inhibition or cell death. Our recent study showed a novel antioxidative mechanism mediated by nitric oxide (NO) in yeast cells
Byung-Man Kwak et al.
Food chemistry, 165, 569-574 (2014-07-21)
A rapid and simple sample preparation method for vitamin D3 (cholecalciferol) was developed for emulsified dairy products such as milk-based infant formulas. A sample was mixed in a 50 mL centrifuge tube with the same amount of water and isopropyl
Yohei Y Yamamoto et al.
Journal of molecular biology, 426(14), 2667-2678 (2014-05-27)
Chaperonins are ubiquitous molecular chaperones with the subunit molecular mass of 60kDa. They exist as double-ring oligomers with central cavities. An ATP-dependent conformational change of the cavity induces the folding of an unfolded protein that is captured in the cavity.
M Venkataramana et al.
Journal of food science and technology, 51(9), 1920-1928 (2014-09-06)
Fumonisins are one of the most agriculturally significant environmental toxins produced by Fusarium and Aspergillus species that grow on agricultural commodities in the field or during storage. Cereals contaminated with fumonisins causes serious loss to agricultural produce leads to health
Francisco Gasteazoro et al.
Analytical biochemistry, 471, 44-50 (2014-12-03)
Ethylene is a key molecule in organic synthesis currently produced by steam cracking of fossil hydrocarbons. In nature, ethylene is produced in higher plants by 1-aminocyclopropane-1-carboxylic acid oxidase (ACCO). Biocatalytic alternatives for ethylene production are still far from being competitive
David A Nagel et al.
Environmental toxicology and pharmacology, 38(3), 968-976 (2014-12-03)
Mixtures of pesticides in foodstuffs and the environment are ubiquitous in the developed world and although agents are usually exhaustively tested individually, the toxicological implications of pesticide mixtures are underreported. In this study, the effects of two fungicides, fenhexamid and
Amr Y Esmat et al.
Pharmaceutical biology, 53(1), 138-146 (2014-09-23)
The antitumor activity of aloin, the active anthraquinone of Aloe juice, against different murine and human tumors has been reported. In the present study, the impact of repeated aloin treatment at its maximum tolerated dose on serum levels of lipid
Juliana Lucena Schussel et al.
Cancer biomarkers : section A of Disease markers, 15(1), 11-17 (2014-12-20)
DACT genes regulates Wnt as well TGF-β pathway, and were already associated with hepatocellular and lung cancer. Alterations on Wnt/β-catenin were associated with head and neck cancer through β-catenin cytoplasmatic accumulation. The aim of the study was to evaluate DACT1
T Shrivastava et al.
Leukemia, 28(10), 2040-2048 (2014-03-22)
Runx1 is required for definitive hematopoiesis and is well known for its frequent chromosomal translocations and point mutations in leukemia. Runx1 regulates a variety of genes via Ets1 activation on an Ets1•Runx1 composite DNA sequence. The structural basis of such
Cheng Jiang et al.
Analytica chimica acta, 766, 88-93 (2013-02-23)
T4 polynucleotide kinase (PNK) plays a critical role in various cellular events. Here, we describe a novel colorimetric strategy for estimating the activity of PNK and screening its inhibitors taking advantage of the efficient cleavage of λ exonuclease and the
Emmanuel Amoakwah et al.
Bulletin of environmental contamination and toxicology, 93(2), 187-191 (2014-06-25)
The use of conventional methods to clean up the soil is very expensive and destructive to the ecosystem. The concept of phytoextraction has been introduced to safely manage soils contaminated with heavy metals. However, using plants to remediate polluted soils
Jana Keller et al.
Archives of toxicology, 88(11), 2033-2059 (2014-10-03)
Two Ceria nanomaterials (NM-211 and NM-212) were tested for inhalation toxicity and organ burdens in order to design a chronic and carcinogenicity inhalation study (OECD TG No. 453). Rats inhaled aerosol concentrations of 0.5, 5, and 25 mg/m(3) by whole-body exposure
Sedigheh Khamehchian et al.
Human vaccines & immunotherapeutics, 10(6), 1633-1638 (2014-03-20)
A combined process of ammonium sulfate precipitation (salting out) and ion-exchange chromatography on DEAE-Sepharose CL-6B was used to prepare camel antivenom (IgG) against Naja Naja Oxiana for therapy. In the ammonium sulfate precipitation, the best condition for fractionation of IgG
Songjia Guo et al.
Journal of agricultural and food chemistry, 63(1), 150-159 (2014-12-20)
Nostoc commune has been traditionally used in China as a health food and medicine. The water stress proteins (WSP) of Nostoc commune are the major component of the extracellular matrix. This study purified and identified the water stress proteins (WSP1)
Sook Shien Lee et al.
BMC complementary and alternative medicine, 14, 359-359 (2014-09-27)
The sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden (Tiger Milk mushroom) is used as a traditional medicine to relieve cough, asthma and chronic hepatitis. The traditional uses of the sclerotium are presumably related to its anti-inflammatory effect. The present study was
Ângela Sousa et al.
Journal of chromatography. A, 1366, 110-119 (2014-09-30)
Histamine and agmatine amino acid derivatives were immobilized into monolithic disks, in order to combine the specificity and selectivity of the ligand with the high mass transfer and binding capacity offered by monolithic supports, to purify potential plasmid DNA biopharmaceuticals.
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