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Steven P D Harborne et al.
Biochimica et biophysica acta, 1847(10), 1245-1253 (2015-07-15)
The mitochondrial ATP-Mg/Pi carrier imports adenine nucleotides from the cytosol into the mitochondrial matrix and exports phosphate. The carrier is regulated by the concentration of cytosolic calcium, altering the size of the adenine nucleotide pool in the mitochondrial matrix in
E D N S Abeyrathne et al.
Poultry science, 92(4), 1091-1097 (2013-03-09)
Ovotransferrin is one of the major egg white proteins that have antimicrobial activity as well as iron binding capability. The objective of this study was to develop a simple and easy method to separate ovotransferrin without using organic solvents. Egg
Xuefeng Peng et al.
Nature microbiology, 6(4), 499-511 (2021-02-03)
The herbivore digestive tract is home to a complex community of anaerobic microbes that work together to break down lignocellulose. These microbiota are an untapped resource of strains, pathways and enzymes that could be applied to convert plant waste into
Elena Bellido et al.
Advanced healthcare materials, 4(8), 1246-1257 (2015-03-17)
The specific modification of the outer surface of the promising porous metal-organic framework nanocarriers (nanoMOFs) preserving their characteristic porosity is still a major challenge. Here a simple, fast, and biofriendly method for the external functionalization of the benchmarked mesoporous iron(III)
Huili Wang et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 985, 62-70 (2015-02-11)
Herein, we developed a novel integrated device to perform phase separation based on ultrasound-assisted salt-induced liquid-liquid microextraction for determination of five fluoroquinones (FQs) in human body fluids. The integrated device consisted of three simple HDPE components used to separate the
Sruthi Purushothaman et al.
Journal of proteomics, 112, 113-124 (2014-09-03)
The extensive arm regeneration of brittle stars following amputation is becoming increasingly recognized as a model system for understanding cellular differentiation and regeneration in a whole animal context. In this study we have used the emerging brittle star model Amphiura
Mervi T Hyvönen et al.
ACS chemical biology, 10(6), 1417-1424 (2015-02-18)
Eukaryotic translation initiation factor 5A (eIF5A) is essential for cell proliferation, becoming functionally active only after post-translational conversion of a specific Lys to hypusine [N(ε)-(4-amino-2-hydroxybutyl)lysine]. Deoxyhypusine synthase (DHS) is the rate-limiting enzyme of this two-step process, and the polyamine spermidine
Tse-Kai Fu et al.
Marine drugs, 17(6) (2019-06-19)
More than 80% of infectious bacteria form biofilm, which is a bacterial cell community surrounded by secreted polysaccharides, proteins and glycolipids. Such bacterial superstructure increases resistance to antimicrobials and host defenses. Thus, to control these biofilm-forming pathogenic bacteria requires antimicrobial
Kazunori Sugiura et al.
Biochemical and biophysical research communications, 457(3), 242-248 (2015-01-17)
Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004))
Joanne Marie M Del Rosario et al.
Frontiers in immunology, 11, 627-627 (2020-06-18)
Cross-subtype neutralizing single domain antibodies against influenza present new opportunities for immunoprophylaxis and pandemic preparedness. Their simple modular structure and single open reading frame format are highly amenable to gene therapy-mediated delivery. We have previously described R1a-B6, an alpaca-derived single
Hui Chan Kwak et al.
Biochemical pharmacology, 96(3), 256-266 (2015-06-07)
The present study was aimed to investigate the metabolomics of sulfur amino acids in Zucker diabetic fatty (ZDF) rats, an obese type 2 diabetic animal model. Plasma levels of total cysteine, homocysteine and methionine, but not glutathione (GSH) were markedly
Virginia Martínez et al.
Applied microbiology and biotechnology, 99(22), 9605-9615 (2015-07-15)
Cloning and biochemical characterization of a novel extracellular medium-chain-length polyhydroxyalkanoate (mcl-PHA) depolymerase from Streptomyces exfoliatus K10 DSMZ 41693 are described. The primary structure of the depolymerase (PhaZSex2) includes the lipase consensus sequence (serine-histidine-aspartic acid) which is known for serine hydrolases.
David P Ward et al.
Journal of chromatography. A, 1411, 84-91 (2015-08-19)
A critical step in the bioprocessing of sustainable biomass feedstocks, such as sugar beet pulp (SBP), is the isolation of the component sugars from the hydrolysed polysaccharides. This facilitates their subsequent conversion into higher value chemicals and pharmaceutical intermediates. Separation
Yukinori Yabuta et al.
Bioscience, biotechnology, and biochemistry, 79(5), 710-717 (2014-12-18)
Methylmalonyl-CoA mutase (MCM) requires 5'-deoxyadenosylcobalamin (AdoCbl) as a cofactor and is widely distributed in organisms from bacteria and animals. Although genes encoding putative MCMs are present in many archaea, they are separately encoded in large and small subunits. The large
T Shrivastava et al.
Leukemia, 28(10), 2040-2048 (2014-03-22)
Runx1 is required for definitive hematopoiesis and is well known for its frequent chromosomal translocations and point mutations in leukemia. Runx1 regulates a variety of genes via Ets1 activation on an Ets1•Runx1 composite DNA sequence. The structural basis of such
Juliana Lucena Schussel et al.
Cancer biomarkers : section A of Disease markers, 15(1), 11-17 (2014-12-20)
DACT genes regulates Wnt as well TGF-β pathway, and were already associated with hepatocellular and lung cancer. Alterations on Wnt/β-catenin were associated with head and neck cancer through β-catenin cytoplasmatic accumulation. The aim of the study was to evaluate DACT1
Jasreen K Sekhon et al.
Bioresource technology, 192, 451-460 (2015-06-17)
Enzyme-assisted aqueous extraction processing (EAEP) is an environmentally-friendly alternative to solvent and mechanical oil extraction methods, and can achieve ∼ 97% oil recovery from soybeans. The present study utilized soy skim (protein rich) and insoluble fiber (IF; carbohydrate rich), both
Sourav Majumdar et al.
International journal of biological macromolecules, 79, 477-489 (2015-05-13)
Fibrin(ogen)olytic enzymes offer great promise for the treatment of thrombosis associated disorders. The present study describes the characterization of an extracellular fibrin(ogen)olytic serine protease (named Bacethrombase) purified from the Bacillus cereus strain FF01. The molecular mass of the Bacethrombase was
Ankita Khanna et al.
Environmental science and pollution research international, 20(8), 5692-5707 (2013-03-07)
Wastewater released from textile industries causes water pollution, and it needs to be treated before discharge to the environment by cost effective technologies. Solar photocatalysis is a promising technology for the treatment of dye wastewater. The Ag@TiO2 nanoparticles comprising of
Janaina de Cássia Orlandi Sardi et al.
Virulence, 6(6), 642-651 (2015-06-10)
Paracoccidioides species are dimorphic fungi that initially infect the lungs but can also spread throughout the body. The spreading infection is most likely due to the formation of a biofilm that makes it difficult for the host to eliminate the
Songjia Guo et al.
Journal of agricultural and food chemistry, 63(1), 150-159 (2014-12-20)
Nostoc commune has been traditionally used in China as a health food and medicine. The water stress proteins (WSP) of Nostoc commune are the major component of the extracellular matrix. This study purified and identified the water stress proteins (WSP1)
Ryo Nasuno et al.
PloS one, 9(11), e113788-e113788 (2014-11-26)
The budding yeast Saccharomyces cerevisiae possesses various defense mechanisms against environmental stresses that generate reactive oxygen species, leading to growth inhibition or cell death. Our recent study showed a novel antioxidative mechanism mediated by nitric oxide (NO) in yeast cells
Hiroko Kobayashi et al.
Biological & pharmaceutical bulletin, 37(11), 1843-1847 (2014-11-05)
Here, we determined the sequence of a cDNA encoding a guanylic acid-specific ribonuclease (RNase He1) from Hericium erinaceus that exhibits high sequence identity (59%) with RNase Po1, an enzyme with anti-cancer activity and which is found in Pleurotus ostreatus. RNase
Sook Shien Lee et al.
BMC complementary and alternative medicine, 14, 359-359 (2014-09-27)
The sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden (Tiger Milk mushroom) is used as a traditional medicine to relieve cough, asthma and chronic hepatitis. The traditional uses of the sclerotium are presumably related to its anti-inflammatory effect. The present study was
Olivier Poch et al.
Science (New York, N.Y.), 367(6483) (2020-03-14)
The measured nitrogen-to-carbon ratio in comets is lower than for the Sun, a discrepancy which could be alleviated if there is an unknown reservoir of nitrogen in comets. The nucleus of comet 67P/Churyumov-Gerasimenko exhibits an unidentified broad spectral reflectance feature
Jana Keller et al.
Archives of toxicology, 88(11), 2033-2059 (2014-10-03)
Two Ceria nanomaterials (NM-211 and NM-212) were tested for inhalation toxicity and organ burdens in order to design a chronic and carcinogenicity inhalation study (OECD TG No. 453). Rats inhaled aerosol concentrations of 0.5, 5, and 25 mg/m(3) by whole-body exposure
Hsueh-Fen Chen et al.
PloS one, 10(6), e0129903-e0129903 (2015-06-19)
Candida albicans is a major human fungal pathogen. One of the important features of C. albicans pathogenicity is the ability to form biofilms on mucosal surfaces and indwelling medical devices. Biofilm formation involves complex processes in C. albicans, including cell
Yohei Y Yamamoto et al.
Journal of molecular biology, 426(14), 2667-2678 (2014-05-27)
Chaperonins are ubiquitous molecular chaperones with the subunit molecular mass of 60kDa. They exist as double-ring oligomers with central cavities. An ATP-dependent conformational change of the cavity induces the folding of an unfolded protein that is captured in the cavity.
Elamaran Meibalan et al.
Eukaryotic cell, 14(4), 371-384 (2015-02-11)
Malaria parasites replicating inside red blood cells (RBCs) export a large subset of proteins into the erythrocyte cytoplasm to facilitate parasite growth and survival. PTEX, the parasite-encoded translocon, mediates protein transport across the parasitophorous vacuolar membrane (PVM) in Plasmodium falciparum-infected
Shang Song et al.
Acta biomaterialia, 18, 100-111 (2015-03-05)
Critical failures associated with current engineered bone grafts involve insufficient induction of osteogenesis of the implanted cells and lack of vascular integration between graft scaffold and host tissue. This study investigated the combined effects of surface microtextures and biochemical supplements
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