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" within Papéis
Muwen Kong et al.
Molecular cell, 79(1), 99-114 (2020-05-24)
Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human
Tris (2-carboxyethyl) phosphine Hydrochloride
Yost Julianne M et al.
Encyclopedia of Reagents for Organic Synthesis, Second Edition, 1-9 (2001)
Tomáš Ječmen et al.
Methods (San Diego, Calif.), 89, 128-137 (2015-08-04)
Protein-protein interactions play a central role in the regulation of many biochemical processes (e.g. the system participating in enzyme catalysis). Therefore, a deeper understanding of protein-protein interactions may contribute to the elucidation of many biologically important mechanisms. For this purpose
Torsten Müller et al.
Molecular systems biology, 16(1), e9111-e9111 (2020-03-05)
High-throughput and streamlined workflows are essential in clinical proteomics for standardized processing of samples from a variety of sources, including fresh-frozen tissue, FFPE tissue, or blood. To reach this goal, we have implemented single-pot solid-phase-enhanced sample preparation (SP3) on a
Yu Wang et al.
Bio-protocol, 10(18), e3767-e3767 (2021-03-05)
B lymphocyte activation is regulated by its membrane-bound B cell receptors (BCRs) upon recognizing diverse antigens. It is hypothesized that antigen binding would trigger conformational changes within BCRs, followed by a series of downstream signaling activation. To measure the BCR
Chiharu Mizuguchi et al.
Biochimica et biophysica acta, 1841(12), 1716-1724 (2014-10-05)
Human apolipoprotein E (apoE) isoforms exhibit different conformational stabilities and lipid-binding properties that give rise to altered cholesterol metabolism among the isoforms. Using Trp-substituted mutations and site- directed fluorescence labeling, we made a comprehensive comparison of the conformational organization of
Leyuan Li et al.
Computational and structural biotechnology journal, 18, 3833-3842 (2020-12-19)
Resistant starches (RS) are dietary compounds processed by the gut microbiota into metabolites, such as butyrate, that are beneficial to the host. The production of butyrate by the microbiome appears to be affected by the plant source and type of
Punam Sonar et al.
Current biology : CB, 30(6), 1160-1166 (2020-03-07)
Construction and function of virtually all cilia require the universally conserved process of intraflagellar transport (IFT) [1, 2]. During the atypically fast IFT in the green alga C. reinhardtii, on average, 10 kinesin-2 motors "line up" in a tight assembly on
Zaur M Kachaev et al.
FEBS letters, 593(10), 1102-1112 (2019-04-20)
The Paip2 protein is a factor regulating mRNA translation and stability in the cytoplasm. It has also been found in the nuclei of several cell types in Drosophila. Here, we aim to elucidate the functions of Paip2 in the cell
Rui Sun et al.
Journal of proteome research, 19(7), 2732-2741 (2020-02-14)
We reported and evaluated a microflow, single-shot, short gradient SWATH MS method intended to accelerate the discovery and verification of protein biomarkers in preclassified clinical specimens. The method uses a 15 min gradient microflow-LC peptide separation, an optimized SWATH MS
Ming Lei et al.
Journal of pharmaceutical sciences, 104(3), 995-1004 (2014-12-30)
The biopharmaceutical industry increasingly demands thorough characterization of protein conformation and conformational dynamics to ensure product quality and consistency. Here, we present a chromatography-based method that is able to characterize protein conformation and conformational dynamics at peptide level resolution in
Matthew S P Boyles et al.
Journal of nanobiotechnology, 13, 84-84 (2015-11-20)
Gold nanoparticles (AuNPs) are a popular choice for use in medical and biomedical research applications. With suitable functionalisation AuNPs can be applied in drug delivery systems, or can aid in disease diagnosis. One such functionalisation is with chitosan, which enables
Dietmar Reusch et al.
mAbs, 7(4), 732-742 (2015-05-23)
To monitor the Fc glycosylation of therapeutic immunoglobulin G in bioprocess development, product characterization and release analytics, reliable techniques for glycosylation analysis are needed. Several analytical methods are suitable for this application. We recently presented results comparing detection methods for
Benjamin D Stein et al.
Cell reports, 29(10), 3331-3348 (2019-12-05)
Metformin is the front-line treatment for type 2 diabetes worldwide. It acts via effects on glucose and lipid metabolism in metabolic tissues, leading to enhanced insulin sensitivity. Despite significant effort, the molecular basis for metformin response remains poorly understood, with
Zsuzsanna Darula et al.
Analytical chemistry, 87(12), 6297-6302 (2015-05-16)
Iodoacetamide is perhaps the most widely used reagent for the alkylation of free sulfhydryls in proteomic experiments. Here, we report that both incomplete derivatization of Cys side chains and overalkylation of the peptides may lead to the misassignment of glycoforms
Sean Chen et al.
Cell reports, 27(13), 3780-3789 (2019-06-27)
Genetically engineered mouse models harboring large sequence insertions or modifications are critical for a wide range of applications including endogenous gene tagging, conditional knockout, site-specific transgene insertion, and gene replacement; however, existing methods to generate such animals remain laborious and
Coordination properties of tris (2-carboxyethyl) phosphine, a newly introduced thiol reductant, and its oxide.
Krezel A, et al.
Inorganic Chemistry, 42(6), 1994-2003 (2003)
Lihua Jiang et al.
Cell, 183(1), 269-283 (2020-09-12)
Determining protein levels in each tissue and how they compare with RNA levels is important for understanding human biology and disease as well as regulatory processes that control protein levels. We quantified the relative protein levels from over 12,000 genes
Isabel Sorg et al.
Cell host & microbe, 27(3), 476-485 (2020-02-27)
Chronically infecting pathogens avoid clearance by the innate immune system by promoting premature transition from an initial pro-inflammatory response toward an anti-inflammatory tissue-repair response. STAT3, a central regulator of inflammation, controls this transition and thus is targeted by numerous chronic
Tatsuya Nobori et al.
Bio-protocol, 8(17), e2987-e2987 (2018-09-05)
Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv. tomato DC3000, in the leaves
Kristy L Hentchel et al.
Journal of bacteriology, 197(2), 314-325 (2014-11-05)
Protein and small-molecule acylation reactions are widespread in nature. Many of the enzymes catalyzing acylation reactions belong to the Gcn5-related N-acetyltransferase (GNAT; PF00583) family, named after the yeast Gcn5 protein. The genome of Salmonella enterica serovar Typhimurium LT2 encodes 26
Emi Takamitsu et al.
Analytical biochemistry, 464, 83-93 (2014-07-22)
To establish a non-radioactive, cell-free detection system for protein N-myristoylation, metabolic labeling in a cell-free protein synthesis system using bioorthogonal myristic acid analogues was performed. After Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) with a biotin tag, the tagged proteins were separated by
James A Bynum et al.
Pharmacology, 95(3-4), 181-192 (2015-05-01)
To identify drugs that may reduce the impact of oxidant stress on cell viability. Human umbilical vein endothelial cells were treated with 200 nmol/l CDDO-Im (imidazole) and CDDO-Me (methyl) after exposure to menadione and compared to vehicle-treated cells. Cell viability
Brad King et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1109, 25-36 (2019-02-02)
Studies of N-acetylcysteine amide (NACA) in nonclinical models have demonstrated various antioxidant, anti-apoptotic, anti-inflammatory and neuroprotective effects, and it is currently being developed as a treatment for retinitis pigmentosa. Sensitive LC-MS/MS methods were developed and validated to quantitate reduced and
A E-L Au et al.
Cell death & disease, 5, e1410-e1410 (2014-09-12)
Platelet activation is a frontline response to injury, not only essential for clot formation but also important for tissue repair. Indeed, the reparative influence of platelets has long been exploited therapeutically where application of platelet concentrates expedites wound recovery. Despite
Erdem Coskun et al.
DNA repair, 33, 101-110 (2015-07-24)
MTH1 protein sanitizes the nucleotide pool so that oxidized 2'-deoxynucleoside triphosphates (dNTPs) cannot be used in DNA replication. Cancer cells require MTH1 to avoid incorporation of oxidized dNTPs into DNA that results in mutations and cell death. Inhibition of MTH1
Purification technique for the removal of ruthenium from olefin metathesis reaction products
Maynard, H. D.; Grubbs, R. J.
Tetrahedron Letters, 40, 4137-4137 (1999)
Multiomics Reveals Ectopic ATP Synthase Blockade Induces Cancer Cell Death via a lncRNA-mediated Phospho-signaling Network.
Chang, et al.
Molecular and Cellular Proteomics, 19, 1805-1825 (2021)
Artem Babaian et al.
Cell reports, 31(5), 107611-107611 (2020-05-07)
The ribosome is an RNA-protein complex that is essential for translation in all domains of life. The structural and catalytic core of the ribosome is its ribosomal RNA (rRNA). While mutations in ribosomal protein (RP) genes are known drivers of
Xixi Zhou et al.
The Journal of biological chemistry, 290(30), 18361-18369 (2015-06-13)
Cysteine oxidation induced by reactive oxygen species (ROS) on redox-sensitive targets such as zinc finger proteins plays a critical role in redox signaling and subsequent biological outcomes. We found that arsenic exposure led to oxidation of certain zinc finger proteins
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