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Protein labeling and modification

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facet applications:Protein labeling and modification
facet content type:Protocol
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Limpeza das reações de marcação de anticorpos utilizando os Amicon Ultra Filters
Utilizando um Amicon® Ultra centrifugal filter padrão, algumas rodadas de diafiltração podem remover eficientemente o rótulo não incorporado de uma reação de rotulagem de anticorpos sem diluir a amostra.
Purification or Removal of Biotin and Biotinylated Biomolecules with Magnetic Beads
This page shows how to Purify or remove biotin and biotinylated biomolecules with magnetic beads.
Tag Removal by Enzymatic Cleavage
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
Clean-up of Antibody Labeling Reactions Using Amicon Ultra Filters
Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.
Removal of GST Tag by Enzymatic Cleavage
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation
CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.
Peptide Quick Tips
Peptide Quick Tips. Helpful guidelines for proper storage and resuspension of your peptide for optimal performance in your assay.
Purification or Removal of Biotin and Biotinylated Substances
This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.
Coupling Protocol for Primary Amine of a Ligand
This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.
Detection of GST-tagged Proteins with SDS-PAGE
This page describes how to monitor GST-tagged protein levels during expression and purification with SDS-PAGE using Cytiva products
Mass Spectrometry of Glycans
The development of modern mass spectrometry (MS) equipment with high resolution and mass accuracy has led to its use in analyzing glycans for both profiling and structural studies.
Conjugating Alkaline Phosphatase to Antibodies and Other Proteins
Protocol for the one-step, glutaraldehyde coupling method to conjugation alkaline phosphatase to antibodies and other proteins.
Coupling to Microparticles
Biochemicals Coupling to Microparticles. Besides Proteins, DNA, lectins and many other species have been coupled to microparticles.
N-Glycosidase F Protocol & Troubleshooting
N-Glycosidase F Protocol & Troubleshooting
Neuraminidase (Sialidase) Protocol
Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.
Enzymatic Deglycosylation
Enzymatic Deglycosylation Strategies: Sequential hydrolysis of individual monosaccharides from glycans can be useful for the elucidation of the structure and function of the glycan component.
Alkaline Phosphatase Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
Exosome Labeling Protocol with PKH Lipophilic Dyes
PKH dyes label exosomes for tracking experiments in vitro and in vivo, detailed protocol provided.