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Protein labeling and modification

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facet applications:Protein labeling and modification
facet content type:Technical Article
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Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
ChiPros Chiral Amines
Chiral amines play an important role in stereoselective organic synthesis. They are used directly as resolving agents, building blocks, or chiral auxiliaries.
Post Translational Modification
Post-translational modifications such as glycosylation, phosphorylation, and sulfation, to name a few, serve many functions. As a result, the analysis of proteins and their post-translational modifications is particularly important for the study of diseases where multiple genes are known to
Solid Phase Synthesis
Peptides are manufactured using solid phase FMOC or BOC chemistry methodologies on a PEG-Polystyrene support resin. Upon synthesis completion, side chain protecting groups are removed and the peptides are simultaneously cleaved from the resin. The cleaved and deprotected
Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity
A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Introduction to Target Identification with Hyas and Dione Kits
In cases where photoaffinity labeling (PAL) probes are ineffective, Hyas and Dione Kits provide an effective method for small molecule identification by using an Iridium photocatalyst tethered to the molecule under investigation.
Phosphorylation Analysis Tools
Phosphorylation is an important covalent post-translational modification (PTM) in cell signalling pathways. Protein phosphorylation is the reversible addition of a phosphate group to a protein or small molecule catalysed by protein kinases. Approximately one third of the 30,000 proteins encoded
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
EX-CELL® Glycosylation Adjust (GAL+)
Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.
Unnatural Amino Acids for Peptide Synthesis
Unnatural amino acids, the non-proteinogenic amino acids that either occur naturally or are chemically synthesized, are becoming more and more important as tools for modern drug discovery research.
Peptidoglycan Structure, Biosynthesis and Function
The basic structure of peptidoglycan (PGN) contains a carbohydrate backbone of alternating units of N-acetylglucosamine (GlcNAc) and Nacetylmuramic acid, with the N-acetylmuramic acid residues cross-linked to peptides.
Glycoprofile™ Labeling Kits
Glycoprofile Labeling Kits for Glycan Analysis designed for efficient labeling of N-linked, O-linked and glycosylphosphatidylinositol (GPI) anchored glycans using your choice of 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA) 1,2
Glycosyltransferases
Glycosyltransferases were initially considered to be specific for a single glycosyl donor and acceptor, which led to the one enzyme-one linkage concept. Subsequent observations have refuted the theory of absolute enzymatic specificity by describing the transfer of analogs of some
Protein Deglycosylation
Protein Deglycosylation: PNGase F Proteomics Grade, Native Protein Deglycosylation Kit, Enzymatic Protein Deglycosylation Kit, and ProteoProfile Trypsin In-Gel Digest Kit
Glycosyltransferases: Tools for Synthesis and Modification of Glycans
The presence of multiple functional groups and stereocenters in complex carbohydrates makes them challenging targets for the organic chemist.
Gold Nanoparticles: Properties and Applications
Gold (Au) nanoparticles have tunable optical and electronic properties and are used in a number of applications including photovoltaics, sensors, drug delivery & catalysis.
Designing Peptides
When selecting peptides for custom synthesis, several important factors should be considered during the design process. These considerations include sequence length, solubility and sequence stability.
Residual Peroxide Determination in Food Packaging
ECM microenvironment shapes cell processes; engineering advances needed for complex control in cell and tissue studies.
O Linked Glycans
O-Linked glycans attachment to peptides involves various residues, particularly GalNAc, known as mucin-type glycans.
Phosphorylation Basics
Explore phosphorylation types converting ADP to ATP, comparing oxidative and substrate-level phosphorylation with explanatory diagrams.
Self-Assembled Monolayers: Advantages of Pure Alkanethiols
We presents an article regarding Self-Assembled Monolayers: Advantages of Pure Alkanethiols.
Methods and Matrices for Mass Spectrometry of Glycans
Glycosylation is known to have profound influence on various physiochemical, cellular and biological functions of proteins. Alterations in this modification are known to affect the immune system and have been associated with various pathological states such as cancer, rheumatoid arthritis
Proline Derivatives and Analogs
Proline analogues are promising candidates for tuning the biological, pharmaceutical, or physicochemical properties of naturally occuring, as well as de novo designed, linear, and, cyclic peptides.
Applications of Y-Shape Peg Derivatives for Drug Delivery
The immune system protects the body from disease by resisting the invasion of foreign molecules into its cells, for example peptides and proteins are hydrolyzed by proteolytic enzymes; nucleic acids are hydrolyzed by nucleases; and most small molecules are eliminated
Chiral Amines in Asymmetric Synthesis
Chiral amines have found widespread application in asymmetric synthesis serving, for instance, as chiral bases in enantioselective deprotonation reactions or being valuable substances for resolving racemic mixtures of acids.
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.
Troubleshooting Strategies in GST-tagged Protein Purification
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
Peptide Stability
When selecting peptides for custom synthesis, several important factors should be considered during the design process. These considerations include sequence length, solubility and sequence stability.
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