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Sandeep Kumar et al.
PloS one, 12(7), e0181235-e0181235 (2017-07-28)
Hypoxia is known to induce pancreatic beta cell dysfunction and apoptosis. Changes in Programmed Cell Death Gene 4 (PDCD4) expression have previously been linked with beta cell neogenesis and function. Our aim was to investigate the effects of hypoxia on
Sebastian Dupraz et al.
Current biology : CB, 29(22), 3874-3886 (2019-11-05)
The specification of an axon and its subsequent outgrowth are key steps during neuronal polarization, a prerequisite to wire the brain. The Rho-guanosine triphosphatase (GTPase) RhoA is believed to be a central player in these processes. However, its physiological role
Bo Ding et al.
Hearing research, 367, 59-73 (2018-07-22)
Due to the critical role of cochlear ion channels for hearing, the focus of the present study was to examine age-related changes of Na, K-ATPase (NKA) subunits in the lateral wall of mouse cochlea. We combined qRT-PCR, western blot and
Héctor Carreño Gutiérrez et al.
Scientific reports, 9(1), 3040-3040 (2019-03-01)
The formation of social groups is an adaptive behaviour that can provide protection from predators, improve foraging and facilitate social learning. However, the costs of proximity can include competition for resources, aggression and kleptoparasitism meaning that the decision whether to
Christopher J Groten et al.
Journal of neurophysiology, 115(5), 2615-2634 (2016-02-13)
After Ca(2+) influx, mitochondria can sequester Ca(2+) and subsequently release it back into the cytosol. This form of Ca(2+)-induced Ca(2+) release (CICR) prolongs Ca(2+) signaling and can potentially mediate activity-dependent plasticity. As Ca(2+) is required for its subsequent release, Ca(2+)
Britney O Pennington et al.
Stem cells translational medicine, 4(2), 165-177 (2015-01-17)
Age-related macular degeneration (AMD), a leading cause of blindness, is characterized by the death of the retinal pigmented epithelium (RPE), which is a monolayer posterior to the retina that supports the photoreceptors. Human embryonic stem cells (hESCs) can generate an
Matthew D Rand et al.
Current protocols in toxicology, 59, 1-1 (2014-05-03)
The fruit fly (Drosophila melanogaster) has long been a premier model for developmental biologists and geneticists. In toxicology studies, Drosophila has only recently gained broader recognition as a tool to elaborate molecular genetic mechanisms of toxic substances. In this article
Yueguang Wei et al.
Molecular medicine reports, 17(2), 2600-2606 (2017-12-06)
Long non‑coding RNA (lncRNA) H19 has been suggested to serve important roles in the progression of gastric cancer (GC); however, the mechanism involved is largely unknown. The present study aimed to investigate the mechanism underlying the effect of H19 on
J D Neves et al.
Brain research bulletin, 134, 55-62 (2017-07-15)
Striatum and the cerebral cortex are regions susceptible to secondary injury after intracerebral hemorrhage (ICH) and glial cells in tissue adjacent to the hematoma may modulate cellular vulnerability after brain damage. Nonetheless, while the glial- associated changes occurring in the
Jiying Shen et al.
Molecular medicine reports, 16(5), 6794-6802 (2017-09-14)
In the current study, it was aimed to evaluate the changes in myelinated and unmyelinated nerve fibers in retinal ischemia‑reperfusion injuries caused by acute ocular hypertension and to determine the sequence of these changes. Adult healthy New Zealand white rabbits
Samuel Hardy et al.
Journal of virology (2020-12-18)
Classical swine fever virus (CSFV) is the causative agent of classical swine fever, a notifiable disease of economic importance that causes severe leukopenia, fever and haemorrhagic disease in domesticated pigs and wild boar across the globe. CSFV has been shown
Emily C Wheeler et al.
Nature methods, 17(6), 636-642 (2020-05-13)
Genetic screens using pooled CRISPR-based approaches are scalable and inexpensive, but restricted to standard readouts, including survival, proliferation and sortable markers. However, many biologically relevant cell states involve cellular and subcellular changes that are only accessible by microscopic visualization, and
Dinorath Olvera et al.
Acta biomaterialia, 64, 148-160 (2017-10-12)
The ideal tissue engineering (TE) strategy for ligament regeneration should recapitulate the bone - calcified cartilage - fibrocartilage - soft tissue interface. Aligned electrospun-fibers have been shown to guide the deposition of a highly organized extracellular matrix (ECM) necessary for
Xiangang Huang et al.
Nature protocols, 17(3), 748-780 (2022-02-06)
Macrophages in atherosclerotic lesions promote plaque progression and are an attractive therapeutic target in cardiovascular research. Here we present a protocol for synthesis of small interfering RNA (siRNA) nanoparticles (NP) that target lesional macrophages as a potential treatment for atherosclerosis.
Mònica Aguilera et al.
International journal of molecular sciences, 22(15) (2021-08-08)
Interactions between the intestinal microbiota, immune system and nervous system are essential for homeostasis in the gut. Inflammasomes contribute to innate immunity and brain-gut interactions, but their role in microbiota-neuro-immune interactions is not clear. Therefore, we investigated the effect of
Raquel V G de Castro et al.
Stem cells international, 2020, 8814989-8814989 (2021-01-19)
The cellular reprogramming into pluripotency is influenced by external and internal cellular factors, such as in vitro culture conditions (e.g., environmental oxygen concentration), and the aging process. Herein, we aimed to generate and maintain equine iPSCs (eiPSCs) derived from fibroblasts
Logan Y Brown et al.
STAR protocols, 1(3), 100152-100152 (2020-12-31)
Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral
Savannah A Lynn et al.
F1000Research, 7, 1107-1107 (2018-10-03)
The retinal pigment epithelium (RPE) plays a key role in the pathogenesis of several blinding retinopathies. Alterations to RPE structure and function are reported in Age-related Macular Degeneration, Stargardt and Best disease as well as pattern dystrophies. However, the precise
Marion Robin et al.
Autophagy, 15(5), 771-784 (2018-12-20)
The tumor suppressor TP53/p53 is a known regulator of apoptosis and macroautophagy/autophagy. However, the molecular mechanism by which TP53 regulates 2 apparently incompatible processes remains unknown. We found that Drosophila lacking p53 displayed impaired autophagic flux, higher caspase activation and
Deanna Bousalis et al.
Frontiers in cardiovascular medicine, 7, 93-93 (2020-06-18)
Congenital heart disease (CHD) affects almost one percent of all live births. Despite diagnostic and surgical reparative advances, the causes and mechanisms of CHD are still primarily unknown. The extracellular matrix plays a large role in cell communication, function, and
Jianjun Chang et al.
Cell biology and toxicology, 36(6), 571-589 (2020-06-27)
Smoke inhalation injury is the leading cause of death in firefighters and victims. Inhaled hot air and toxic smoke are the predominant hazards to the respiratory epithelium. We aimed to analyze the effects of thermal stress and smoke aldehyde on
Michael A Wheeler et al.
Cell, 176(3), 581-596 (2019-01-22)
Genome-wide studies have identified genetic variants linked to neurologic diseases. Environmental factors also play important roles, but no methods are available for their comprehensive investigation. We developed an approach that combines genomic data, screens in a novel zebrafish model, computational
Zhigang Shi et al.
eLife, 9 (2020-06-17)
Whether leptin acts in the paraventricular nucleus (PVN) to increase sympathetic nerve activity (SNA) is unclear, since PVN leptin receptors (LepR) are sparse. We show in rats that PVN leptin slowly increases SNA to muscle and brown adipose tissue, because
Lynn Yap et al.
Cell reports, 26(12), 3231-3245 (2019-03-21)
Regeneration of injured human heart muscle is limited and an unmet clinical need. There are no methods for the reproducible generation of clinical-quality stem cell-derived cardiovascular progenitors (CVPs). We identified laminin-221 (LN-221) as the most likely expressed cardiac laminin. We
O Dubourg et al.
Acta myologica : myopathies and cardiomyopathies : official journal of the Mediterranean Society of Myology, 30(2), 103-108 (2011-11-24)
Sporadic inclusion body myositis (s-IBM) is characterized histologically by the association of concomitant inflammatory and degenerative processes. We evaluated the sensitivity and specificity of different markers of the degenerative process in order to refine the histological diagnosis. We performed an
Lexiang Li et al.
American journal of translational research, 13(10), 11026-11047 (2021-11-18)
Tissue engineering is widely applied to treat osteochondral damage in osteoarthritis (OA). However, the superposition of seed cells, material scaffolds, inducing factors, and microenvironmental factors limit their practical application. We intended to develop a novel tissue engineering method for improving
Teresa H Wen et al.
Cerebral cortex (New York, N.Y. : 1991), 28(11), 3951-3964 (2017-10-19)
Abnormal sensory responses associated with Fragile X Syndrome (FXS) and autism spectrum disorders include hypersensitivity and impaired habituation to repeated stimuli. Similar sensory deficits are also observed in adult Fmr1 knock-out (KO) mice and are reversed by genetic deletion of
Tatsuo S Okubo et al.
Neuron, 107(5), 924-940 (2020-07-19)
Spatial maps in the brain are most accurate when they are linked to external sensory cues. Here, we show that the compass in the Drosophila brain is linked to the direction of the wind. Shifting the wind rightward rotates the
Laina Freyer et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 239(6), 1708-1722 (2010-05-27)
To understand the mechanism by which canonical Wnt signaling sets boundaries for pattern formation in the otic vesicle (OV), we examined Tbx1 and Eya1-Six1 downstream of activated beta-catenin. Tbx1, the gene for velo-cardio-facial syndrome/DiGeorge syndrome (VCFS/DGS), is essential for inner
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