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Atsushi Mukaiyama et al.
International journal of biological macromolecules, 131, 67-73 (2019-03-13)
KaiC, the core protein of the cyanobacterial clock, assembles into a hexamer upon ATP-binding. The hexameric KaiC from a cyanobacterium Synechococcus elongatus PCC 7942 (Se-KaiC) is a multifunctional enzyme with autokinase, autophosphatase and ATPase and these activities show a circadian
Xiukun Wang et al.
Development (Cambridge, England), 146(13) (2019-06-14)
Epigenetic regulation, including histone-to-protamine exchanges, controls spermiogenesis. However, the underlying mechanisms of this regulation are largely unknown. Here, we report that PHF7, a testis-specific PHD and RING finger domain-containing protein, is essential for histone-to-protamine exchange in mice. PHF7 is specifically
Diego Jurado-Bruggeman et al.
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 124(2), 302-310 (2017-07-09)
We performed a multi-centre intercomparison of VMAT dose planning and pre-treatment verification. The aims were to analyse the dose plans in terms of dosimetric quality and deliverability, and to validate whether in-house pre-treatment verification results agreed with those of an
Vanessa Aust et al.
Cytokine, 111, 246-254 (2018-09-11)
Pneumococcal meningitis, caused by Streptococcus pneumoniae, is the most common type of bacterial meningitis. The clinical management of this disease has been challenged by the emergence of multidrug-resistant Streptococcus pneumoniae, requiring the urgent development of new therapeutic alternatives. Over the
Sadiye Hayta et al.
Plant methods, 14, 93-93 (2018-11-06)
Genetic transformation is a valuable tool and an important procedure in plant functional genomics contributing to gene discovery, allowing powerful insights into gene function and genetically controlled characteristics. Primulaceae species provide one of the best-known examples of heteromorphic flower development
B W Raaymakers et al.
Physics in medicine and biology, 62(23), L41-L50 (2017-11-15)
The integration of 1.5 T MRI functionality with a radiotherapy linear accelerator (linac) has been pursued since 1999 by the UMC Utrecht in close collaboration with Elekta and Philips. The idea behind this integrated device is to offer unrivalled, online
Ondřej Novák et al.
The Plant journal : for cell and molecular biology, 72(3), 523-536 (2012-06-26)
The plant hormone auxin is believed to influence almost every aspect of plant growth and development. Auxin transport, biosynthesis and degradation combine to form gradients of the hormone that influence a range of key developmental and environmental response processes. There
Carlos Roberto Sanquetta et al.
Carbon balance and management, 13(1), 20-20 (2018-10-24)
We analyzed the dynamics of carbon (C) stocks and CO2 removals by Brazilian forest plantations over the period 1990-2016. Data on the extent of forests compiled from various sources were used in the calculations. Productivities were simulated using species-specific growth
Qiang Su et al.
Science (New York, N.Y.), 361(6406) (2018-08-11)
Mutations in two genes, PKD1 and PKD2, account for most cases of autosomal dominant polycystic kidney disease, one of the most common monogenetic disorders. Here we report the 3.6-angstrom cryo-electron microscopy structure of truncated human PKD1-PKD2 complex assembled in a
Nikolay Vasilev et al.
Scientific reports, 6, 37390-37390 (2016-11-18)
Multiple factors act simultaneously on plants to establish complex interaction networks involving nutrients, elicitors and metabolites. Metabolomics offers a better understanding of complex biological systems, but evaluating the simultaneous impact of different parameters on metabolic pathways that have many components
Emna Fourati et al.
Chemosphere, 219, 463-471 (2018-12-15)
Sesuvium portulacastrum, a halophyte with high tolerance to heavy metals like Cd, Pb and Ni is considered for phytoremediation of metal contaminated saline soils. The tolerance to a selected metal ion could, by hypothesis, be stimulated through in vitro adaptation and
Karthikeyan Alagarsamy et al.
3 Biotech, 8(8), 356-356 (2018-08-15)
Explant oxidative browning and necrosis of Camellia sinensis var. sinensis is a severe problem in tissue culture, often associated with the exuded phenolic compounds and microbial contamination from the explants. In this study, 2-aminoindane-2-phosphonic acid (AIP), an inhibitor of the
Josiane Fukami et al.
AMB Express, 7(1), 153-153 (2017-07-21)
Azospirillum spp. are plant-growth-promoting bacteria used worldwide as inoculants for a variety of crops. Among the beneficial mechanisms associated with Azospirillum inoculation, emphasis has been given to the biological nitrogen fixation process and to the synthesis of phytohormones. In Brazil
Mei-Ling Kuo et al.
Scientific reports, 6, 18846-18846 (2016-01-07)
Ribonucleotide reductase small subunit B (RRM2B) is a stress response protein that protects normal human fibroblasts from oxidative stress. However, the underlying mechanism that governs this function is not entirely understood. To identify factors that interact with RRM2B and mediate
Chun-Hua Wu et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 37(24), 3768-3772 (2013-05-01)
To investigate the effect of auxins 2,4-D,IAA,IBA,NAA on induction of adventitious roots as well as that of IBA concentrations on the growth of adventitious roots and the accumulation of caffeic acid derivatives, with test-tube seedling leaves Echinacea pallida as the
Laura Airas et al.
Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 56(2), 305-310 (2015-01-13)
There is a great need for the monitoring of microglial activation surrounding multiple sclerosis lesions because the activation of microglia is thought to drive widespread neuronal damage. Recently, second-generation PET radioligands that can reveal the extent of microglial activation by
Chenyu Yan et al.
Journal of applied clinical medical physics, 18(5), 195-209 (2017-08-24)
The main aim of this study is to validate the Acuros XB dose calculation algorithm for a Varian Clinac iX linac in our clinics, and subsequently compare it with the wildely used AAA algorithm. The source models for both Acuros
Zhiguo Liu et al.
BMC genomics, 18(1), 855-855 (2017-11-11)
Chinese jujube (Ziziphus jujuba Mill.) is one of the most important members in the Rhamnaceae family. The whole genome sequence and more than 30,000 proteins of Chinese jujube have been obtained in 2014. Mitogen-activated protein kinase cascades are universal signal
Fausto López-Rodríguez et al.
Journal of environmental management, 190, 45-52 (2017-01-01)
Protected areas are home to biodiversity, habitats and ecosystem as well as a critical component of human well-being and a generator of leisure-related revenues. However, management is sometimes unsatisfactory and requires new ways of evaluation. Management effectiveness of 36 protected
Linjing Mu et al.
ACS chemical neuroscience, 7(11), 1552-1564 (2016-08-30)
Serotonin-gated ionotropic 5-HT
Marvin Rausch et al.
Nature communications, 10(1), 1404-1404 (2019-03-31)
The Gram-positive cell wall consists of peptidoglycan functionalized with anionic glycopolymers, such as wall teichoic acid and capsular polysaccharide (CP). How the different cell wall polymers are assembled in a coordinated fashion is not fully understood. Here, we reconstitute Staphylococcus
Wenli Zhu et al.
Neurology(R) neuroimmunology & neuroinflammation, 6(3), e561-e561 (2019-05-03)
Using phage display, we sought to screen single-chain variable fragments (scFvs) against complement C5 to treat neuromyelitis optica spectrum disorder (NMOSD). After 5 rounds of phage display, we isolated individual clones and identified phage clones specifically binding to C5 using
Milena Trajković et al.
3 Biotech, 9(1), 17-17 (2019-01-10)
The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C
Vida Mildažienė et al.
Scientific reports, 9(1), 6437-6437 (2019-04-25)
Treatment of plant seeds with electromagnetic fields or non-thermal plasmas aims to take advantage of plant functional plasticity towards stimulation of plant agricultural performance. In this study, the effects of pre-sowing seed treatment using 200 Pa vacuum (7 min), 5.28 MHz radio-frequency cold
Syandan Sinha Ray et al.
World journal of microbiology & biotechnology, 33(2), 31-31 (2017-01-08)
Bambusa balcooa is an economically important, multipurpose bamboo species, decidedly used in construction industry. Availability of natural bamboo is depleting very rapidly due to accelerated deforestation and its unrestrained use. The large number and timely supply of saplings are the
Amit Ketkar et al.
ACS chemical biology, 14(6), 1337-1351 (2019-05-15)
Overexpression of human DNA polymerase kappa (hpol κ) in glioblastoma is associated with shorter survival time and resistance to the alkylating agent temozolomide (TMZ), making it an attractive target for the development of small-molecule inhibitors. We previously reported on the
Shuai Wang et al.
The Journal of cell biology, 216(11), 3639-3653 (2017-09-21)
Protein targeting to the bacterial plasma membrane was generally thought to occur via two major pathways: cotranslational targeting by signal recognition particle (SRP) and posttranslational targeting by SecA and SecB. Recently, SecA was found to also bind ribosomes near the
Stefanie Ronzheimer et al.
Frontiers in microbiology, 9, 2536-2536 (2018-11-09)
Accumulation of compatible solutes is a common stress response of microorganisms challenged by high osmolarity; it can be achieved either through synthesis or import. These processes have been intensively studied in Bacillus subtilis, where systems for the production of the
Sensen Zhang et al.
Protein & cell, 8(11), 834-847 (2017-09-25)
TRPML1 channel is a non-selective group-2 transient receptor potential (TRP) channel with Ca
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