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José F Huertas-Pérez et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 32(8), 1352-1357 (2015-07-01)
A rapid, simple and effective method for the determination of citrinin in rice by UHPLC coupled to fluorescence detection has been developed and validated. Extraction of citrinin from rice samples was achieved by applying a QuEChERS-based extraction/partitioning process with water
Lilia C De Jesus et al.
American journal of obstetrics and gynecology, 212(1), 94-94 (2014-07-22)
Antenatal magnesium (anteMg) is used for various obstetric indications including fetal neuroprotection. Infants exposed to anteMg may be at risk for respiratory depression and delivery room (DR) resuscitation. The study objective was to compare the risk of acute cardiorespiratory events
Woo-Yong Lee et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(38), 13936-13941 (2014-09-11)
CXCR6-GFP(+) cells, which encompass 70% invariant natural killer T cells (iNKT cells), have been found primarily patrolling inside blood vessels in the liver. Although the iNKT cells fail to interact with live pathogens, they do respond to bacterial glycolipids presented
Xi Zhan et al.
Pharmaceutical research, 32(7), 2280-2291 (2015-01-17)
A key step of delivering extracellular agents to its intracellular target is to escape from endosomal/lysosomal compartments, while minimizing the release of digestive enzymes that may compromise cellular functions. In this study, we examined the intracellular distribution of both fluorecent
Sharmistha Samanta Koruri et al.
Archives of microbiology, 197(7), 955-963 (2015-07-15)
The present investigation deals with the optimization of cell growth rate of the candidate probiotic Pediococcus acidilactici in the presence of the specific prebiotic inulin. Three independent variables viz. concentration of inulin, concentration of glucose and pH have been selected
Yi Wang et al.
International journal of urology : official journal of the Japanese Urological Association, 21(10), 1059-1064 (2014-05-23)
To investigate the effect of diabetes on urothelial modulation of bladder contractility. Bladder strips (urothelium intact or denuded) were prepared from 8-week-old streptozotocin-induced diabetic (n = 19) and non-diabetic control rats (n = 10). The effect of modulators of MaxiK (iberiotoxin and tetraethylammonium) and
Yuko Matsushita et al.
Journal of microbiological methods, 112, 11-20 (2015-03-01)
Variation in band position between gels is a well-known problem in denaturing gradient gel electrophoresis (DGGE). However, few reports have evaluated the degree of variation in detail. In this study, we investigated the variation in band positions of DNA samples
Michalina Oplatowska-Stachowiak et al.
Journal of agricultural and food chemistry, 63(43), 9441-9451 (2015-10-10)
There are more than 300 potential mycotoxins that can contaminate food and feed and cause adverse effects in humans and animals. The data on the co-occurrence of mycotoxins in novel animal feed materials, such as distiller's dried grain with solubles
Anna Gadaj et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 960, 105-115 (2014-05-06)
An UHPLC-MS/MS method was developed for the quantitative confirmatory analysis of residues of nitroimidazole drugs (dimetridazole, ipronidazole, metronidazole, ornidazole and ronidazole) and their corresponding hydroxy metabolites (HMMNI, ipronidazile-OH and metronidazole-OH) in aquaculture tissue. Samples were extracted by shaking in acetonitrile
Christoph Czarnetzki et al.
Anesthesiology, 121(1), 59-67 (2014-03-13)
Magnesium enhances the effect of rocuronium. Sugammadex reverses rocuronium-induced neuromuscular block. The authors investigated whether magnesium decreased the efficacy of sugammadex for the reversal of rocuronium-induced neuromuscular block. Thirty-two male patients were randomized in a double-blinded manner to receive magnesium
Viviane A V N Braga et al.
PloS one, 10(4), e0125388-e0125388 (2015-04-30)
Previous studies have shown that estrogen deficiency, arising in postmenopause, promotes endothelial dysfunction. This study evaluated the effects of aerobic exercise training on endothelial dependent vasodilation of aorta in ovariectomized rats, specifically investigating the role of nitric oxide (NO) and
Patricia Scheans
Neonatal network : NN, 31(2), 121-124 (2012-03-09)
Magnesium sulfate (MgSO4) has been widely used in the perinatal arena for many decades. It has been used for tocolysis in the U.S. for more than 60 years. Estimations of MgSO4 use for preterm labor (less than 34 weeks of
Agustín Conde-Agudelo et al.
American journal of obstetrics and gynecology, 209(6), 551-551 (2013-07-31)
The purpose of this study was to evaluate the efficacy and safety of transdermal nitroglycerin as a tocolytic agent in women with preterm labor. We conducted a systematic review and metaanalysis of randomized controlled trials. Thirteen studies were included (1302
Masahiro Watanabe et al.
AMB Express, 4, 27-27 (2014-06-21)
Cellulosic materials constitute most of the biomass on earth, and can be converted into biofuel or bio-based materials if fermentable sugars can be released using cellulose-related enzymes. Acremonium cellulolyticus is a mesophilic fungus which produces a high amount of cellulose-related
Robert J Floor et al.
Chembiochem : a European journal of chemical biology, 15(11), 1660-1672 (2014-07-01)
We explored the use of a computational design framework for the stabilization of the haloalkane dehalogenase LinB. Energy calculations, disulfide bond design, molecular dynamics simulations, and rational inspection of mutant structures predicted many stabilizing mutations. Screening of these in small
Daniel J Modulevsky et al.
PloS one, 9(5), e97835-e97835 (2014-05-21)
There are numerous approaches for producing natural and synthetic 3D scaffolds that support the proliferation of mammalian cells. 3D scaffolds better represent the natural cellular microenvironment and have many potential applications in vitro and in vivo. Here, we demonstrate that
Hiromi Murase et al.
Investigative ophthalmology & visual science, 56(4), 2511-2518 (2015-03-26)
Renewal and elimination of the aged photoreceptor outer segment (POS) by RPE cells is a daily rhythmic process that is important for long-term vision. Phagocytic dysfunction results in photoreceptor cell death. Tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, is known
Afrouz Yousefi et al.
Pharmaceutical research, 31(11), 3127-3135 (2014-05-21)
Since the discovery of RNAi and its therapeutic potential, carrier systems have been developed to deliver small RNAs (particularly siRNA) for modulation of gene expression at the post-transcriptional level. An important factor determining the fate and usability of these systems
Shuxin Yang et al.
BMC genomics, 16, 547-547 (2015-07-26)
Although the responses to many pathogen associated molecular patterns (PAMPs) in cell cultures and extracted organs are well characterized, there is little known of transcriptome responses to PAMPs in whole organisms. To characterize this in detail, we have performed RNAseq
R Zagrodnik et al.
Bioresource technology, 200, 1039-1043 (2015-11-26)
The role of light intensity on biohydrogen production from glucose by Clostridium beijerinckii, Clostridium acetobutylicum, and Rhodobacter sphaeroides was studied to evaluate the performance and possible application in co-culture fermentation system. The applied source of light had spectrum similar to
Jan E Janecka et al.
The Journal of experimental biology, 218(Pt 15), 2402-2409 (2015-08-08)
Genetically based modifications of hemoglobin (Hb) function that increase blood-O2 affinity are hallmarks of hypoxia adaptation in vertebrates. Among mammals, felid Hbs are unusual in that they have low intrinsic O2 affinities and reduced sensitivities to the allosteric cofactor 2,3-diphosphoglycerate
Lei Yu et al.
Journal of neural transmission (Vienna, Austria : 1996), 122(6), 747-755 (2014-11-06)
Orexin deficiency results in cataplexy, a motor deficit characterized by sudden loss of muscle tone, strongly indicating an active role of central orexinergic system in motor control. However, effects of orexin on neurons in central motor structures are still largely
Guohua Gong et al.
American journal of physiology. Heart and circulatory physiology, 309(7), H1166-H1177 (2015-08-16)
Mitochondrial respiration through electron transport chain (ETC) activity generates ATP and reactive oxygen species in eukaryotic cells. The modulation of mitochondrial respiration in vivo or under physiological conditions remains elusive largely due to the lack of appropriate approach to monitor
Pierina Visciano et al.
Journal of food protection, 78(9), 1719-1728 (2015-09-01)
Concentrations of pollutants with regulatory limits were determined in specimens of Chamelea gallina, a species of clam collected along the Abruzzi coastal region of the central Adriatic Sea. Nine sampling sites were selected to evaluate the distribution of contaminants in
Nora Semren et al.
PloS one, 10(9), e0136188-e0136188 (2015-09-05)
Proteasome inhibition has been shown to prevent development of fibrosis in several organs including the lung. However, effects of proteasome inhibitors on lung fibrosis are controversial and cytotoxic side effects of the overall inhibition of proteasomal protein degradation cannot be
Arya Haj-Mirzaian et al.
Psychopharmacology, 232(19), 3551-3561 (2015-07-16)
The antidepressant action of acute nicotine administration in clinical and animal studies is well recognized. But the underlying mechanism for this effect has not been carefully discovered. We attempted to evaluate the possible role of N-Methyl-D-aspartate (NMDA) receptors in the
Mónica García López et al.
Journal of chromatography. A, 1373, 40-50 (2014-12-04)
This study reports the development and validation of a screening method for the detection of pesticides in 11 different fruit and vegetable commodities. The method was based on ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-QTOF-MS). The objective was to
Duy Luong et al.
Analytica chimica acta, 879, 48-57 (2015-05-24)
Subcritical water extraction of organic matter containing sedimentary rocks at 300°C and 1500 psi produces extracts comparable to conventional solvent extraction. Subcritical water extraction of previously solvent extracted samples confirms that high molecular weight organic matter (kerogen) degradation is not
Helena Lindgren et al.
PloS one, 10(3), e0119143-e0119143 (2015-03-11)
Francisella tularensis is a highly virulent facultative intracellular pathogen causing the severe disease tularemia in mammals. As for other bacteria, iron is essential for its growth but very few mechanisms for iron acquisition have been identified. Here, we analyzed if
Silvana Constantinescu et al.
Canadian journal of physiology and pharmacology, 93(11), 913-922 (2015-09-26)
We have shown that reduced expression of receptor-interacting protein 140 (RIP140) alters the regulation of fatty-acid (FA) oxidation in muscle. To determine whether a high level of FA availability alters the effects of RIP140 on metabolic regulation, L6 myotubes were
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